RT Journal Article
SR Electronic
T1 EFFECT OF OUABAIN ON GLYCEROL-EXTRACTED FIBERS FROM HEART CONTAINING A "RELAXING FACTOR"
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 149
OP 155
VO 132
IS 2
A1 Lee, Kwang Soo
YR 1961
UL http://jpet.aspetjournals.org/content/132/2/149.abstract
AB Heart muscle fibers extracted at -20°C in 50% glycerol with 0.1 M histidine buffer at pH 7.00 for a short duration (approximately 3 to 15 days) were found to contain a "relaxing factor." This "relaxing factor" was not present in fibers extracted or preincubated for a prolonged time (over 3 weeks). Ouabain increased the ATP-induced contraction of these glycerol-extracted fibers containing the "relaxing factor." This action of ouabain occurred only after a 20-minute contact with the fiber. This effect of ouabain was not seen when the "relaxing factor" was absent from the fibers. Thus ouabain was effective only in those fibers which contracted in response to Ca++.