RT Journal Article
SR Electronic
T1 A SIMPLIFIED MICROMETHOD FOR THE DETERMINATION OF CARBONIC ANHYDRASE AND ITS INHIBITORS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 26
OP 29
VO 130
IS 1
A1 Thomas H. Maren
YR 1960
UL http://jpet.aspetjournals.org/content/130/1/26.abstract
AB A simple quantitative micromethod for carbonic anhydrase based on the changing pH principle is described. Sensitivity is such that the activity of 0.05 ml of mammalian blood diluted 1:1000 can be detected. The method is suitable for estimating the activity of carbonic anhydrase inhibitors and for their determination in biological material. For acetazolamide, the limit of sensitivity is 0.2 µg/g tissue. The nature of carbonic anhydrase inhibition by aromatic sulfonamides is briefly discussed, as it may relate to certain variables in the analytical method.