PT  - JOURNAL ARTICLE
AU  - Maren, Thomas H.
TI  - A SIMPLIFIED MICROMETHOD FOR THE DETERMINATION OF CARBONIC ANHYDRASE AND ITS INHIBITORS
DP  - 1960 Sep 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 26--29
VI  - 130
IP  - 1
4099  - http://jpet.aspetjournals.org/content/130/1/26.short
4100  - http://jpet.aspetjournals.org/content/130/1/26.full
SO  - J Pharmacol Exp Ther1960 Sep 01; 130
AB  - A simple quantitative micromethod for carbonic anhydrase based on the changing pH principle is described. Sensitivity is such that the activity of 0.05 ml of mammalian blood diluted 1:1000 can be detected. The method is suitable for estimating the activity of carbonic anhydrase inhibitors and for their determination in biological material. For acetazolamide, the limit of sensitivity is 0.2 µg/g tissue. The nature of carbonic anhydrase inhibition by aromatic sulfonamides is briefly discussed, as it may relate to certain variables in the analytical method.