RT Journal Article
SR Electronic
T1 BIOCHEMICAL ALTERATIONS OF LIVER FUNCTION BY THE HALOGENATED HYDROCARBONS. I. <em>IN VITRO</em> AND <em>IN VIVO</em> CHANGES AND THEIR MODIFICATION BY ETHYLENEDIAMINE TETRAACETATE
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 273
OP 281
VO 124
IS 4
A1 Deane N. Calvert
A1 Theodore M. Brody
YR 1958
UL http://jpet.aspetjournals.org/content/124/4/273.abstract
AB In vitro treatment of liver mitochondria with carbon tetrachloride results in a depression of DPN-linked dehydrogenase activity. The addition of DPN partially restores oxidation. Oxidative phosphorylation is inhibited, but this effect is probably the result of a magnesium ATP-ase activation. The oxidation of choline is enhanced. These changes can be blocked by the concomitant addition of Versene. The same general pattern in oxidation and phosphorylation is observed with the oral administration of carbon tetrachloride. In addition changes are seen in tissue sodium and potassium concentrations and in serum glutamic-oxaloacetic transaminase activity. All effects are modified by Versene administered simultaneously. Choline administration failed to protect against the metabolic changes induced by carbon tetrachloride. There did not appear to be any correlation between metabolic alterations and histologic findings. The possible mechanisms by which Versene protects are discussed.