PT  - JOURNAL ARTICLE
AU  - James L. Spratt
AU  - George T. Okita
TI  - SUBCELLULAR LOCALIZATION OF RADIOACTIVE DIGITOXIN
DP  - 1958 Oct 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 115--119
VI  - 124
IP  - 2
4099  - http://jpet.aspetjournals.org/content/124/2/115.short
4100  - http://jpet.aspetjournals.org/content/124/2/115.full
SO  - J Pharmacol Exp Ther1958 Oct 01; 124
AB  - The essential experimental findings of this study are listed as follows. Twenty minutes after a single intravenous injection, the rat essentially clears its vascular system of digitoxin with a significant amount of metabolism of the parent compound. Upon cell fractionation of in vivo preparations, over 90% of the unchanged digitoxin present in the cell is found in the soluble supernate of rat heart, kidney and liver cells. No marked quantitative binding could be demonstrated with any of the particulate fractions. The results of the in vitro experiments agree with the in vivo work. Washing of the particulate fractions does lower the binding capacities of these fractions, but does not change the gross distribution picture. The presence or absence of nonradioactive drug in the suspending media exerts no significant effect on the binding distribution.