PT  - JOURNAL ARTICLE
AU  - Bogdanski, Donald F.
AU  - Pletscher, Alfred
AU  - Brodie, Bernard B.
AU  - Udenfriend, Sidney
TI  - IDENTIFICATION AND ASSAY OF SEROTONIN IN BRAIN
DP  - 1956 May 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 82--88
VI  - 117
IP  - 1
4099  - http://jpet.aspetjournals.org/content/117/1/82.short
4100  - http://jpet.aspetjournals.org/content/117/1/82.full
SO  - J Pharmacol Exp Ther1956 May 01; 117
AB  - 1. A fluorometric method for the determination of serotonin (5-hydroxytryp-tamine) in brain is described. The procedure involves the use of a spectrophoto-fluorometer which can activate and measure emitted fluorescence continuously from 250 to 650 mµ. Serotonin is activated maximally at 295 mµ and emits fluorescent light which shows a maximum at 550 mµ. 2. With this method serotonin has been shown to be a normal constituent of brain. 3. The fluorescent procedure may be applied to the measurement of serotonin in other tissues.