RT Journal Article
SR Electronic
T1 THE HISTOCHEMICAL LOCALIZATION OF ACETYL-CHOLINESTERASE IN NERVOUS TISSUE
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 37
OP 53
VO 107
IS 1
A1 Herbert A. Ravin
A1 Sumner I. Zacks
A1 Arnold M. Seligman
YR 1953
UL http://jpet.aspetjournals.org/content/107/1/37.abstract
AB A new method is described for the histochemical localization of acetylcholinesterase in fresh frozen sections of nervous tissue, based upon the acetyl-esterase function of this enzyme. Evidence by means of specific inhibitors is given that staining in nervous tissue is due mainly to acetylcholinesterase activity. Enzymatic activity was demonstrated in the cytoplasm and cell membranes of neurocytes, in the neuropile and in myoneural junctions. Cell nuclei were free of enzymatic activity. Illustrations are given.