PT  - JOURNAL ARTICLE
AU  - Herbert A. Ravin
AU  - Sumner I. Zacks
AU  - Arnold M. Seligman
TI  - THE HISTOCHEMICAL LOCALIZATION OF ACETYL-CHOLINESTERASE IN NERVOUS TISSUE
DP  - 1953 Jan 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 37--53
VI  - 107
IP  - 1
4099  - http://jpet.aspetjournals.org/content/107/1/37.short
4100  - http://jpet.aspetjournals.org/content/107/1/37.full
SO  - J Pharmacol Exp Ther1953 Jan 01; 107
AB  - A new method is described for the histochemical localization of acetylcholinesterase in fresh frozen sections of nervous tissue, based upon the acetyl-esterase function of this enzyme. Evidence by means of specific inhibitors is given that staining in nervous tissue is due mainly to acetylcholinesterase activity. Enzymatic activity was demonstrated in the cytoplasm and cell membranes of neurocytes, in the neuropile and in myoneural junctions. Cell nuclei were free of enzymatic activity. Illustrations are given.