PT  - JOURNAL ARTICLE
AU  - Ya-jie Xiong
AU  - Ying Zhu
AU  - Ya-li Liu
AU  - Yi-fan Zhao
AU  - Xiao Shen
AU  - Wen-qing Zuo
AU  - Fang Lin
AU  - Zhong-qin Liang
TI  - P300 Participates in Ionizing Radiation–Mediated Activation of Cathepsin L by Mutant p53
AID  - 10.1124/jpet.121.000639
DP  - 2021 Sep 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 276--286
VI  - 378
IP  - 3
4099  - http://jpet.aspetjournals.org/content/378/3/276.short
4100  - http://jpet.aspetjournals.org/content/378/3/276.full
SO  - J Pharmacol Exp Ther2021 Sep 01; 378
AB  - Our previous studies have shown that cathepsin L (CTSL) is involved in the ability of tumors to resist ionizing radiation (IR), but the specific mechanisms responsible for this remain unknown. We report here that mutant p53 (mut-p53) is involved in IR-induced transcription of CTSL. We found that irradiation caused activation of CTSL in mut-p53 cell lines, whereas there was almost no activation in p53 wild-type cell lines. Additionally, luciferase reporter gene assay results demonstrated that IR induced the p53 binding region on the CTSL promoter. We further demonstrated that the expression of p300 and early growth response factor-1 (Egr-1) was upregulated in mut-p53 cell lines after IR treatment. Accordingly, the expression of Ac-H3, Ac-H4, AcH3K9 was upregulated after IR treatment in mut-p53 cell lines, whereas histone deacetylase (HDAC) 4 and HDAC6 were reciprocally decreased. Moreover, knockdown of either Egr-1 or p300 abolished the binding of mut-p53 to the promoter of CTSL. Chromatin immunoprecipitation assay results showed that the IR-activated transcription of CTSL was dependent on p300. To further delineate the clinical relevance of interactions between Egr-1/p300, mut-p53, and CTSL, we accessed primary tumor samples to evaluate the relationships between mut-p53, CTSL, and Egr-1/p300 ex vivo. The results support the notion that mut-p53 is correlated with CTSL transcription involving the Egr-1/p300 pathway. Taken together, the results of our study revealed that p300 is an important target in the process of IR-induced transcription of CTSL, which confirms that CTSL participates in mut-p53 gain-of-function.SIGNIFICANCE STATEMENT Transcriptional activation of cathepsin L by ionizing radiation required the involvement of mutated p53 and Egr-1/p300. Interference with Egr-1 or p300 could inhibit the expression of cathepsin L induced by ionizing radiation. The transcriptional activation of cathepsin L by p300 may be mediated by p53 binding sites on the cathepsin L promoter.