RT Journal Article
SR Electronic
T1 <strong>A novel mitochondrial complex of P450c11AS, StAR and Tom22</strong><strong> synthesizes aldosterone in the rat heart</strong>
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP JPET-AR-2020-000365
DO 10.1124/jpet.120.000365
A1 Bose, Himangshu S.
A1 Whittal, Randy M
A1 Marshall, Brendan
A1 Rajapaksha, Maheshinie S
A1 Wang, Ning Ping
A1 Bose, Madhuchanda
A1 Perry, Elizabeth W
A1 Zhao, Zhi-Qing
A1 Miller, Walter L
YR 2021
UL http://jpet.aspetjournals.org/content/early/2021/02/01/jpet.120.000365.abstract
AB Aldosterone, which regulates renal salt retention, is synthesized in adrenocortical mitochondria in response to angiotensin II. Excess aldosterone causes myocardial injury and heart failure, but potential intra-cardiac aldosterone synthesis has been controversial. We hypothesized that the stressed heart might produce aldosterone. We used blue native gel electrophoresis, immunoblotting, protein crosslinking, co-immunoprecipitations and mass spectrometry to assess rat cardiac aldosterone synthesis. Chronic infusion of angiotensin II increased circulating corticosterone levels 350-fold and induced cardiac fibrosis. AngII doubled, and telmisartan inhibited, aldosterone synthesis by heart mitochondria, and cardiac production of aldosterone synthase (P450c11AS). Heart aldosterone synthesis required P450c11AS, Tom22 (a mitochondrial translocase receptor), and the intramitochondrial form of the steroidogenic acute regulatory protein (StAR); protein crosslinking and co-immunoprecipitation studies showed these three proteins form a 110-kDa complex. In steroidogenic cells, extramitochondrial (37-kDa) StAR promotes cholesterol movement from the outer to inner mitochondrial membrane where P450scc (cholesterol side-chain cleavage enzyme) converts cholesterol to pregnenolone, thus initiating steroidogenesis, but no function has previously been ascribed to intramitochondrial (30-kDa) StAR; our data indicate that intramitochondrial 30-kDa StAR is required for aldosterone synthesis in the heart, forming a tri-molecular complex with Tom22 and P450c11AS. This is the first activity ascribed to intramitochondrial StAR, but how this promotes P450c11AS activity is unclear. The stressed heart did not express P450scc, suggesting that circulating corticosterone (rather than intracellular cholesterol) is the substrate for cardiac aldosterone synthesis. Thus, the stressed heart produced aldosterone using a previously undescribed intramitochondrial mechanism that involves P450c11AS, Tom22 and 30-kDa StAR. Significance Statement Prior studies of potential cardiac aldosterone synthesis have been inconsistent. We show that the stressed rat heart produces aldosterone by a novel mechanism involving P450c11AS, Tom22 and intramitochondrial StAR apparently using circulating corticosterone as substrate. This study establishes that the stressed rat heart produces aldosterone, and, for the first time, identifies a biological role for intramitochondrial 30-kDa StAR.