%0 Journal Article %A Kathryn Braden %A Luigino Antonio Giancotti %A Zhoumou Chen %A Chelsea DeLeon %A Nick Latzo %A Terri Boehm %A Napoleon D'Cunha %A Bonne M. Thompson %A Timothy M. Doyle %A Jeffrey G. McDonald %A John K. Walker %A Grant R. Kolar %A Christopher Kent Arnatt %A Daniela Salvemini %T GPR183-oxysterol axis in spinal cord contributes to neuropathic pain %D 2020 %R 10.1124/jpet.120.000105 %J Journal of Pharmacology and Experimental Therapeutics %P JPET-AR-2020-000105 %X Neuropathic pain is a debilitating public health concern for which novel non-narcotic therapeutic targets are desperately needed. Using unbiased transcriptomic screening of the dorsal horn spinal cord after nerve injury we have identified that Gpr183 (Epstein-Barr induced gene 2 [EBI2]) is upregulated after chronic constriction injury (CCI) in rats. GPR183 is a chemotactic receptor known for its role in the maturation of B cells and the endogenous ligand is the oxysterol, 7α,25-dihydroxycholesterol (7α,25-OHC). The role of GPR183 in the central nervous system is not well characterized and its role in pain is unknown. The profile of commercially available probes for GPR183 limits their use as pharmacological tools to dissect the roles of this receptor in pathophysiological settings. Using in silico modeling, we have screened a library of 5 million compounds to identify several novel small-molecule antagonists of GPR183 with nanomolar potency. These compounds are able to antagonize 7α,25-OHC-induced calcium mobilization in vitro with IC50 values below 50nM. In vivo intrathecal injections of these antagonists during peak pain after CCI surgery reversed allodynia in male and female mice. Acute intrathecal injection of the GPR183 ligand, 7α,25-OHC in naïve mice induced dose-dependent allodynia. Importantly, this effect was blocked using our novel GPR183 antagonists, suggesting spinal GPR183 activation as pro-nociceptive. These studies are the first to reveal a role for GPR183 in neuropathic pain and identify this receptor as a potential target for therapeutic intervention. Significance Statement We have identified several novel GPR183 antagonists with nanomolar potency. Using these antagonists, we have demonstrated that GPR183 signaling in the spinal cord is pro-nociceptive. These studies are the first to reveal a role for GPR183 in neuropathic pain and identify it as a potential target for therapeutic intervention. %U https://jpet.aspetjournals.org/content/jpet/early/2020/09/10/jpet.120.000105.full.pdf