Abstract
Characteristics of the uptake mechanisms for 3-o-methyl glucose (3mG) or 2-deoxyglucose (2dG) by guinea-pig seminal vesicles (S.V.) and anterior lobes of the prostate gland were investigated in vitro. Neither 3mG nor free 2dG (nonphosphorylated) were able to reach an equilibrium with tissue water and therefore were not concentrated in sex accessory organs. Substantial amounts of 2dG were phosphorylated by S.V., but these organs were unable to convert 3mG to 3mGPO4. Increasing the concentration of nonradioactive 2dG in the medium led to significant reductions in the percent space of total 2dG-14C. This reduction in the total fraction was due principally to the phosphorylation of this nonutilizable hexose and suggested that this effect was due to partial saturation of hexokinase or at least some phosphorylating mechanism. S.V. incubated in the presence of phiorizin (1.5 x 10-2 M) showed a significant reduction in the percent space for both total 2dG and 2dGPO4. Free 2dG and 3mG percent spaces were not affected by this inhibitor. Lowering the temperature to 20°C caused a reduction in the percent space of both total 2dG and 2dGPO4. The 2°C environment had no effect on the free 2dG or 3mG percent space. 3mG and free 2dG are probably incorporated into sex accessory organs by the process of simple diffusion, but their metabolic fates are different.
Footnotes
- Received November 2, 1970.
- Accepted January 3, 1971.
- © 1971 by The Williams & Wilkins Co.
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