Abstract
The anaerobic metabolism of mitomycin C by rat liver homogenates and cell fractions was described. A TPNH-dependent system was localized in the microsomal (37000 x g) fraction. A more complex system, requiring DPN+, malate, ATP, and Mg++, was fully active only when mitochondria, microsomes and supernatant were recombined. When homogenates were supplemented with a TPNH-generating system no requirement for ATP, Mg++ or nicotinamide was evident although ATP had a sparing action with low concentrations of TPN+. Chemical reduction decreased toxicity in mice and antibiotic activity against B. subtilis.
Footnotes
- Received December 15, 1961.
JPET articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.