Abstract
The data show that two components of blood sulfhydryl, measured by methods devised for GSH and SH, behaved differently under certain experimental conditions: the concentration of the latter increased while that of the former decreased. This behavior occurred during methemoglobin formation, taking place both in vivo and in vitro when a direct methemoglobin-former (nitrite) was used, but only in vivo when an indirectly-acting agent (PAPP) was used. Some quantitative parallelism between the formation of methemoglobin and the formation of new SH groups was demonstrated. Alloxan, a poor methemoglobin former, significantly reduced the concentration of blood GSH without appreciably affecting the SH level.
Footnotes
- Received December 29, 1953.
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