Abstract
Anwulignan is one of monomer compounds in the lignans from Schisandra sphenanthera. In this study, we observed the effect of anwulignan on the intestinal ischemia/reperfusion (II/R) injury in male SD rats and explored the underlying mechanisms. The results showed that the pretreatment with oral anwulignan could significantly increase the mesenteric blood microcirculatory flow velocity; relieve the congestion and pathological injury of jejunum; enhance the autonomic tension of jejunum smooth muscle and its reactivity to acetylcholine; increase the activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and choline acetyltransferase (ChAT); increase the contents of acetylcholine (ACh) and glutathione (GSH) in the serum or jejunal tissue; decrease the activities of myeloperoxidase (MPO), protein kinase C (PKC), and nicotinamide adenine dinucleotide phosphate oxidase (NOX); reduced the contents of malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), nicotinamide adenine, reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), interlukin-6 (IL-6), and interlukin-1 β (IL-1 β); increased the expression levels of M3 receptor, PI3K, p-AKT, p-GSK3β Ser9, Nrf2, p-Nrf2, HO-1 and Bcl2 in the jejunal tissue decreased the expression levels of p-GSK3β Tyr216, Keap1, Bax and Cleaved Caspase-3, suggesting that anwulignan can ameliorate the II/R-induced jejunal tissue injury in rats, and the mechanism may be related to its activating the PI3K/AKT pathway and then the to regulate Nrf2/ARE signaling pathway and the expression of apoptosis-related proteins to play antioxidant and antiapoptotic roles.
Significance Statement Anwulignan can significantly reduce the jejunal tissue injury and the production of inflammatory factors in rats with intestinal ischemia-reperfusion injury, improve the antioxidant capacity, reduce the apoptosis of jejunal tissue, and has the effect of significantly improving the intestinal ischemia-reperfusion injury in rats, suggesting that anwulignan may be used as a potential drug for the prevention and treatment of intestinal ischemia-reperfusion injury or a resource for the development of health food.
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