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Journal of Pharmacology and Experimental Therapeutics

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OtherBehavioral Pharmacology

Rapid throughput concentration-response analysis of human taste discrimination

R. Kyle Palmer, Mariah M Stewart and John Talley
Journal of Pharmacology and Experimental Therapeutics January 19, 2021, JPET-AR-2020-000373; DOI: https://doi.org/10.1124/jpet.120.000373
R. Kyle Palmer
1Opertech Bio, Inc., United States of America
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  • For correspondence: kpalmer@opertechbio.com
Mariah M Stewart
2Research and Development, Opertech Bio, Inc., United States of America
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John Talley
2Research and Development, Opertech Bio, Inc., United States of America
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Additional Files

  • Data Supplement

    • Supplemental Figures -

      Figure S1: 96-well plate configuration for training sessions.

      Figure S2: 96-well plate configuration for Experiment 1 test sessions.

      Figure S3: 96-well plate configuration for Experiment 2A-2C test sessions.

      Figure S4: 96-well plate configuration for Experiment 3 test sessions.

      Figure S5: 96-well plate configuration for Experiment 4 test sessions.

      Figure S6: Total number of errors occurring on each of the 96 trials from a dataset summated across subjects from 226 training sessions.

    • Supplemental Video 1 -

      SUPPLEMENTAL VIDEO S1 LEGEND. Operations of the sample delivery system. Frontabove
      view of the sample delivery system; the lid of the cabinet housing the x-y motion table is
      removed. At the beginning of a trial, the x-y motion table moves to the wash station position and
      the pipette tip is lowered into the water-filled Eppendorf tube for a wash cycle. Water is
      withdrawn from and dispended back into the tube once. Then, the pipette is raised above the
      plate, and the 96-well plate immediately is moved to a position aligning a single randomly
      selected well (containing taste stimulus solution) with the pipette tip. The pipetted is lowered so
      that the tip enters the well to withdraw 200 μl of solution.
      Commercial logos are covered.

    • Supplemental Video 2 -

      SUPPLEMENTAL VIDEO S2 LEGEND. Responses recorded on the touch-sensitive
      display and consequences to the responses. At the beginning of the first segment of the
      video clip, the 8x12 matrix appearing to the right of the response field initially shows that 18
      trials have occurred, the first 4 of which were errors (touch responses occurring outside of the
      target designated for the training cue or control standard on that trial, indicated by red “X”s in
      the cells of the matrix). Each of the errors had been penalized by a reduction of 10 points
      ($0.10) from the cumulative score; the 5th trial was a correct response, but slightly off-target
      from the central coordinates, and thus had been reinforced with a red, $0.10 poker chip. The
      following 13 trials had been correct responses centered within the “bullseye” (each reinforced
      with blue, $0.20 poker chips). The lower left ticker beneath the matrix indicates that 78 trials
      remain, and the ticker on the lower right of the matrix shows that the current score is 230 ((13 x
      20 + 10) – (4 x 10), the equivalent to $2.30). The next response—correct—occasions a positive
      reinforcer (blue, $0.20 poker chip and “cha-ching” sound), raising the score to 250 ($2.50). Both
      tickers adjust accordingly.
      By the start of the next segment of the video, an additional (20th) trial has already happened,
      that trial having resulted in an error (red “X” in the 20th cell of the matrix). The next response
      also is an error and occasions a penalty—point reduction of 10, buzzer and timeout.
      Commercial logos are covered.

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Journal of Pharmacology and Experimental Therapeutics: 381 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 381, Issue 3
1 Jun 2022
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OtherBehavioral Pharmacology

Concentration-response analysis human taste discrimination

R. Kyle Palmer, Mariah M Stewart and John Talley
Journal of Pharmacology and Experimental Therapeutics January 19, 2021, JPET-AR-2020-000373; DOI: https://doi.org/10.1124/jpet.120.000373

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OtherBehavioral Pharmacology

Concentration-response analysis human taste discrimination

R. Kyle Palmer, Mariah M Stewart and John Talley
Journal of Pharmacology and Experimental Therapeutics January 19, 2021, JPET-AR-2020-000373; DOI: https://doi.org/10.1124/jpet.120.000373
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