Abstract
1. Phenol disappears when added to guinea pig liver slices in Ringer bicarbonate solution shaken at 37° in 95% oxygen 5% carbon dioxide. 20-30% of the amount that disappears is conjugated.
2. Thermolabile enzyme systems are responsible for the disappearance and conjugation. Neither process occurs anaerobically. The effects of the amount of tissue and substrate concentration are described.
3. In the absence of added sulfate no conjugation occurs. Cystine or methionine cannot act as sources of sulfate under the experimental conditions.
4. M/300 cocaine HCl inhibits the disappearance of phenol but has no effect on the percentage conjugated.
Footnotes
- Received April 21, 1943.
JPET articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.