Abstract
Wieland's bufotalin does not appear to occur in the secretion or skin of Bufo bufo bufo (B. vulgaris).
The three chief principles we isolated are:
(a) Cholesterol which is admixed with ergosterol in the proportion of 4.7 parts per thousand, as proved spectrographically.
(b) Vulgaro-bufotoxin, C38H60O11N4, which has a digitalis-like action, as shown by the myocardiogram, electrocardiogram, and the emetic action. The minimal emetic dose in pigeons is 0.2, that in cats 0.125, and the average fatal dose in 10 cats is 0.3 mgm. per kilogram. Its cardiac effect does not seem to be persistent. It has a pressor action due to constriction of blood vessels, and a stimulating action on the isolated intestines and uteri.
(c) Vulgaro-bufotenine, in the form of a flavianate, C12H18O2N2·-C10H6O8N2S, which contains an indole ring. It increases the tone of cardiac contractions in frogs as illustrated by perfusion, and stimulates the isolated intestines and uterus in dilute solutions, with prompt recovery. It raises the blood pressure in pithed cats with small doses (0.2 to 0.25 mgm.). Its pressor activity is on an average 58 per cent that of cino-bufotenine.
Footnotes
- Received July 11, 1932.
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