Abstract
1. A frog method for the biological assay of digitalis and strophanthus preparations is described in detail.
2. The method eliminates the effect of individual variation in the frogs and variations which occur from time to time.
3. The results of a series of assays on tinctures of digitalis and strophanthus are presented.
4. The official U. S. P. X method is discussed in comparison with the method described. The wide fluctuations in the M. S. D. values for ouabain are attributed to neglect of the effect of individual differences in the sensitivity of the frogs.
5. Seasonal, diurnal and species variations are discussed.
6. It is maintained that the frog method, using the technique described, combines accuracy, simplicity and economy to a greater degree than other methods.
Footnotes
- Received March 14, 1932.
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