Abstract
1. The assay of gonad-stimulating principles (hebin) by the use of immature male and female rats is discussed. The method used is based on the weight of the seminal vesicles in the male, and the ovaries in the female.
2. The preparation of a crude pituitary hebin from various sources is discussed. It is pointed out that while beef anterior lobes are a poor source for this substance, good yields can be obtained from sheep and pork pituitary bodies.
3. Pituitary hebin, administered subcutaneously, can initiate oestrus in the hypophysectomized rat. Associated with oestrus, there occur follicular maturation and uterine changes.
4. Crude pituitary hebin does not dialyze through collodion or parchment; it is heat stabile (99°C.). It may be extracted from pituitary bodies at both acid and alkaline pHs.
5. Hebin from pregnancy-urine, does not dialyze through collodion or parchment; it deteriorates when boiled in aqueous solution.
6. Urinary hebin stimulates the testis and ovary with equal facility; pituitary hebin, on the other hand, stimulates the ovary in a much smaller dose than that having a moderate effect on the testis.
Footnotes
- Received April 24, 1931.
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