Abstract
It has been proposed that inhaled E-prostanoid 4 (EP4)-receptor agonists could represent a new class of bronchodilators for the treatment of asthma that are as effective as β2-adrenoceptor agonists. However, the genomic impact of such drugs is unknown despite being potentially deleterious to respiratory health. Herein, we used mRNA-seq to compare the transcriptomic responses produced by 2-[3-[(1R,2S,3R)-3-hydroxy-2-[(E,3S)-3-hydroxy-5-[2-(methoxymethyl)phenyl]pent-1-enyl]-5-oxo-cyclopentyl]sulphanylpropylsulphanyl] acetic acid (ONO-AE1-329; an EP4-receptor agonist) and vilanterol (a β2-adrenoceptor agonist) in BEAS-2B human airway epithelial cells. We also determined if an increase in cAMP mediated by different G protein-coupled receptors (GPCRs) promoted distinct transcriptional signatures by expanding this inquiry to include the adenosine A2B- and I-prostanoid receptor agonists, 2-[[6-amino-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]-2-pyridinyl]thio]-acetamide (Bay60-6583) and taprostene, respectively. Maximally-effective concentrations of ONO-AE1-329 and vilanterol significantly regulated (q ≤ 0.05; ≥1.5-/≤0.67-fold) 232 and 320 genes, respectively of which 217 were shared. Spearman analysis showed these gene expression changes to be highly rank order correlated, indicating that the functional overlap between the two interventions should be considerable. Unexpectedly, the genomic effects of ONO-AE1-329, vilanterol, Bay 60-6583, and taprostene were also highly rank order correlated. This finding suggests that cAMP generated by any GPCR would initiate the same transcriptional program. Nevertheless, relative to vilanterol, ONO-AE1-329 typically behaved as a partial agonist that varied across transcripts. These data indicate that each ONO-AE1-329-regulated gene differs in sensitivity to cAMP and is defined by a unique receptor occupancy-response relationship. Moreover, if this relatively modest genomic response in BEAS-2B cells is retained in vivo, then inhaled EP4-receptor agonists could represent an alternative, and possibly safer, class of bronchodilators.
SIGNIFICANCE STATEMENT The genomic consequences of β2-adrenoceptor agonists in asthma are often overlooked despite being potentially harmful to lung health. We determined that ONO-AE1-329, an EP4-receptor agonist and effective bronchodilator, produced gene expression changes in BEAS-2B cells that were typically modest relative to the β2-adrenoceptor agonist vilanterol. Furthermore, ONO-AE1-329 behaved as a partial agonist that varied across transcripts. If this genomic activity is reproduced in vivo, then EP4-receptor agonists could represent an alternative, and possibly safer, class of bronchodilators.:
Footnotes
- Received March 14, 2024.
- Accepted July 17, 2024.
This study was supported by the Canadian Institutes for Health Research (PJT 152904) and the Natural Sciences and Engineering Research Council (Discovery Grant, RGPIN-2018-04312). O.H. was a recipient of a studentship awarded by The Lung Association of Alberta and NWT. D.Y. was supported by Alberta Innovates. Real-time PCR was facilitated by an equipment and infrastructure grant from the Canadian Fund of Innovation and the Alberta Science and Research Authority.
The authors state no conflict of interest.
↵1Current affiliation: Deltek, Alberta, Canada.
↵2Current affiliation: Northern RNA, Inc., Alberta, Canada.
↵3Current affiliation: Deep Apple Therapeutics, South San Francisco, California.
↵4Current affiliation: Ted Rogers Centre for Heart Research, Translational Biology and Engineering Program, University of Toronto, Ontario, Canada.
↵This article has supplemental material available at jpet.aspetjournals.org.
- Copyright © 2024 by The American Society for Pharmacology and Experimental Therapeutics
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