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Review ArticleSpecial Section on Non-Coding RNAs in Clinical Practice: From Biomarkers to Therapeutic Tools—Minireview

The Multiplicity of Argonaute Complexes in Mammalian Cells

Maurizio Mauro, Massimiliano Berretta, Giuseppe Palermo, Vincenzo Cavalieri and Gaspare La Rocca
Journal of Pharmacology and Experimental Therapeutics January 2023, 384 (1) 1-9; DOI: https://doi.org/10.1124/jpet.122.001158
Maurizio Mauro
Department of Medicine, Albert Einstein College of Medicine, New York, New York (M.M.); Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy (M.B.); Gruppo Oncologico Ricercatori Italiani, GORI ONLUS, Pordenone, Italy (M.B.); Department of Biomedical and Biotechnological Sciences, University of Catania, Catania Italy (G.P.); Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy (V.C.); and Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, New York (G.L.R.)
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  • ORCID record for Maurizio Mauro
Massimiliano Berretta
Department of Medicine, Albert Einstein College of Medicine, New York, New York (M.M.); Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy (M.B.); Gruppo Oncologico Ricercatori Italiani, GORI ONLUS, Pordenone, Italy (M.B.); Department of Biomedical and Biotechnological Sciences, University of Catania, Catania Italy (G.P.); Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy (V.C.); and Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, New York (G.L.R.)
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Giuseppe Palermo
Department of Medicine, Albert Einstein College of Medicine, New York, New York (M.M.); Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy (M.B.); Gruppo Oncologico Ricercatori Italiani, GORI ONLUS, Pordenone, Italy (M.B.); Department of Biomedical and Biotechnological Sciences, University of Catania, Catania Italy (G.P.); Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy (V.C.); and Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, New York (G.L.R.)
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Vincenzo Cavalieri
Department of Medicine, Albert Einstein College of Medicine, New York, New York (M.M.); Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy (M.B.); Gruppo Oncologico Ricercatori Italiani, GORI ONLUS, Pordenone, Italy (M.B.); Department of Biomedical and Biotechnological Sciences, University of Catania, Catania Italy (G.P.); Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy (V.C.); and Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, New York (G.L.R.)
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  • ORCID record for Vincenzo Cavalieri
Gaspare La Rocca
Department of Medicine, Albert Einstein College of Medicine, New York, New York (M.M.); Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy (M.B.); Gruppo Oncologico Ricercatori Italiani, GORI ONLUS, Pordenone, Italy (M.B.); Department of Biomedical and Biotechnological Sciences, University of Catania, Catania Italy (G.P.); Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy (V.C.); and Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, New York (G.L.R.)
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    Fig. 1.

    Mechanisms of post-transcriptional gene repression mediated by the RISC. miRNAs guide the RISC to partially complementary binding sites within the 3′ UTR of a target mRNA. Next, decapping and deadenylase complexes are recruited on the target mRNA. Upon deadenylation and decapping, mRNAs become substrate for cellular exonucleases and are rapidly degraded.

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    Fig. 2.

    SEC-based analysis of RISC size in whole cell and tissue lysates. Whole cell lysates are prepared as described in the main text, cleared by centrifugation, and loaded on a fast protein liquid chromatography automated system. One-milliliter fractions are collected, processed for trichloroacetic acid precipitation, and analyzed by Western blotting for detection of RISC components.

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Journal of Pharmacology and Experimental Therapeutics: 384 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 384, Issue 1
1 Jan 2023
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Review ArticleSpecial Section on Non-Coding RNAs in Clinical Practice: From Biomarkers to Therapeutic Tools—Minireview

The Multiplicity of AGO Complexes

Maurizio Mauro, Massimiliano Berretta, Giuseppe Palermo, Vincenzo Cavalieri and Gaspare La Rocca
Journal of Pharmacology and Experimental Therapeutics January 1, 2023, 384 (1) 1-9; DOI: https://doi.org/10.1124/jpet.122.001158

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Review ArticleSpecial Section on Non-Coding RNAs in Clinical Practice: From Biomarkers to Therapeutic Tools—Minireview

The Multiplicity of AGO Complexes

Maurizio Mauro, Massimiliano Berretta, Giuseppe Palermo, Vincenzo Cavalieri and Gaspare La Rocca
Journal of Pharmacology and Experimental Therapeutics January 1, 2023, 384 (1) 1-9; DOI: https://doi.org/10.1124/jpet.122.001158
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