Fig. 3. Effects of valsartan and sacubitril, alone or in combination, on AngII-induced AAAs. Mice were infused with AngII (1000 ng/kg per minute) in the absence (vehicle) or presence of valsartan (0.3, 0.5, 1, 6, or 20 mg/kg per day), sacubitril (1, 6, or 9 mg/kg per day), or combinations thereof (0.3/1; 0.5/9 valsartan/sacubitril). (A) Abdominal aortic internal lumen diameters (day 28 of infusions) were decreased dose-dependently by valsartan, whereas sacubitril had no effect alone or in combination with valsartan. (B) AAA external diameters were decreased dose-dependently by valsartan, with no effect of sacubitril alone or in combination with valsartan. For (A) and (B), data are individual mice with means ± S.D. indicated by horizontal lines from vehicle (total of 51 mice, of which 31 mice survived the infusion protocol), valsartan 0.3 (total of 19 mice, of which 11 mice survived the infusion protocol), valsartan 0.5 (total of eight mice, of which six mice survived the infusion protocol), valsartan 1 (total of eight mice, of which seven mice survived the infusion protocol), valsartan 6 (total of eight mice, of which eight mice survived the infusion protocol), valsartan 20 (total of eight mice, of which eight mice survived the infusion protocol), sacubitril 1 (total of 19, of which 11 survived the infusion protocol), sacubitril 6 (total of 10, of which seven mice survived the infusion protocol), valsartan 0.3/sacubitril 1 (total of nine mice, of which five mice survived the infusion protocol), and valsartan 0.5/sacubitril 9 (total of eight mice of which six mice survived the infusion protocol). (C) AAA incidence (percentage) was decreased dose-dependently by valsartan, with no effect of sacubitril alone or in combination with valsartan. Data are individual mice with means ± S.D. indicated by horizontal lines from vehicle (n = 51), valsartan 0.3 (n = 19), valsartan 0.5 (n = 8), valsartan 1 (n = 8), valsartan 6 (n = 8), valsartan 20 (n = 8), sacubitril 1 (n = 19), sacubitril 6 (n = 10), valsartan 0.3/sacubitril 1 (n = 9), and valsartan 0.5/sacubitril 9 (n = 8). (D) Representative aortas from mice of each treatment group. *P < 0.05 compared with vehicle by one-way ANOVA, Kruskal-Wallis, or Fisher’s exact test.Sacubitril, Sac; Valsartan, Val.