Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleDrug Discovery and Translational Medicine

Clostridium butyricum Alleviates Gut Microbiota Alteration–Induced Bone Loss after Bariatric Surgery by Promoting Bone Autophagy

Xueying Shang, Xiaolei Zhang, Cen Du, Zhuoqi Ma, Shi Jin, Na Ao, Jing Yang and Jian Du
Journal of Pharmacology and Experimental Therapeutics May 2021, 377 (2) 254-264; DOI: https://doi.org/10.1124/jpet.120.000410
Xueying Shang
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaolei Zhang
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cen Du
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhuoqi Ma
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shi Jin
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Na Ao
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jing Yang
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jian Du
Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jian Du
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Body weight alterations after bariatric surgery. (A) The experimental design is presented as a flowchart. SD rats were randomized to have RYGB or SG surgery, and body weight alterations were measured every week (B). Data are expressed as means ± S.D. of n = 6. Compared with the RYGBS group: #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001; compared with SGS group: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

  • Fig. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Effect of bariatric surgery on bone metabolism in rats. (A) Whole-body bone density was determined by using DXA. (B) The trabecular bone microstructure of tibia in each group was determined by micro-CT with the analysis of BV/TV, Tb. Th, Tb. N, Tb.Sp, and SMI. (C) The pathologic alteration of each group was determined by H&E staining. Scale bar, 100 μm. The activities of osteoblasts and osteoclasts in each group were respectively analyzed by ALP staining (D) and TRAP staining (E). Scale bar, 100 μm. (F) The content of bone collagen of each group was assessed by Sirius Red staining. Scale bar, 100 μm. (G) The expression levels of RANKL and OPG were detected by immunohistochemical staining. Scale bar, 50 μm. Data are expressed as means ± S.D. of n = 6. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

  • Fig. 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Effect of bariatric surgery on osteoblast autophagy in rats. (A) The LC3II/I, p62, and beclin 1 expression levels were analyzed by Western blot. (B) The expression level of beclin 1 in each group was determined by immunohistochemical staining. Black arrow: beclin 1–positive osteoblasts. Scale bar, 100 μm. (C) Quantitative analysis of beclin 1–positive osteoblasts. Data are expressed as means ± S.D. of n = 6. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

  • Fig. 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 4.

    Alterations of the gut microbiome after bariatric surgery. (A) The Venn diagram shows the similarities and overlap between four groups. (B) The Shannon index suggests α diversity among different groups. (C) The weighted PCoA plot analysis indicates the β diversity in each group. (D) The difference between different phyla is presented by heatmap. (E) Relative abundance analysis of top four bacterial phyla. (F) The genus-level differences in different groups is presented by heatmap. (G) Relative abundance analysis of the top eight differentiated bacterial genera. Data are expressed as means ± S.D. of n = 5. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

  • Fig. 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 5.

    Body weight alterations after the RYGB fecal microbiota transplantation and C. butyricum intervention. (A) The experimental design is presented as a flowchart. Obese rats received RYGB fecal microbiota transplantation and C. butyricum intervention. The body weight changes were recorded every week after manipulation (B). Data are expressed as means ± S.D. of n = 6. Compared with the sham D group: #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001; compared with RYGBD group: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

  • Fig. 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 6.

    Effect of C. butyricum on RYGB fecal microbiota transplantation–induced impaired bone metabolism in pseudo germ-free obese rats. (A) Whole-body bone density was determined by using DXA. (B) The images of tibia trabecular microstructure with micro-CT analyses in different groups. (C) The pathologic alterations in each group were determined by H&E staining. (D) The osteoblast activity was presented as ALP staining. Scale bar, 100 μm. (E–F) TARP and Sirius Red staining were respectively performed to analyze the activity of osteoclasts and collagen content. Scale bar, 100 μm. (G) The expression levels of RANKL and OPG were determined by immunohistochemical staining. Scale bar, 50 μm. Data are expressed as means ± S.D. of n = 6. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

  • Fig. 7.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 7.

    Effect of C. butyricum on RYGB fecal microbiota transplantation–induced defective osteoblast autophagy in pseudo germ-free obese rats. (A–C) Western blot was conducted to measure the difference in protein levels of LC3II/I, p62, and beclin 1. (D) Immunohistochemical staining was performed to show the changes in beclin 1 expression. Black arrow: beclin 1–positive osteoblasts. Scale bar, 100 μm. (E) Quantitative analysis of beclin 1–positive osteoblasts. Data are expressed as means ± S.D. of n = 6. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Tables

  • Figures
    • View popup
    TABLE 1

    Effect of surgery on the bone turnover markers

    All values are expressed as means ± S.D. (n = 6).

    ParametersRYGBSRYGBSGSSG
    CTX-I (pg/ml)1266 ± 2084385 ± 96####1027 ± 952836 ± 276***
    OCN (pg/ml)2091.37 ± 110.331256.92 ± 100.65###1924.35 ± 144.461008.33 ± 126.73**
    • #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001, compared with RYGBS group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, compared with SGS group.

    • View popup
    TABLE 2

    Effect of RYGB fecal microbiota intervention and C. butyricum administration on bone turnover markers

    All values are expressed as means ± S.D. (n = 6).

    TestsSham DRYGB DRYGB D + C.B
    CTX-I (pg/ml)2250 ± 835098 ± 452####2492 ± 208****
    OCN (pg/ml)1405.41 ± 78.751103.63 ± 170.22#1686.99 ± 84.20**
    • #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001, compared with Sham D group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, compared with RYGB D group.

PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 377 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 377, Issue 2
1 May 2021
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Clostridium butyricum Alleviates Gut Microbiota Alteration–Induced Bone Loss after Bariatric Surgery by Promoting Bone Autophagy
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleDrug Discovery and Translational Medicine

C. butyricum Alleviates Bone Loss after Bariatric Surgery

Xueying Shang, Xiaolei Zhang, Cen Du, Zhuoqi Ma, Shi Jin, Na Ao, Jing Yang and Jian Du
Journal of Pharmacology and Experimental Therapeutics May 1, 2021, 377 (2) 254-264; DOI: https://doi.org/10.1124/jpet.120.000410

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleDrug Discovery and Translational Medicine

C. butyricum Alleviates Bone Loss after Bariatric Surgery

Xueying Shang, Xiaolei Zhang, Cen Du, Zhuoqi Ma, Shi Jin, Na Ao, Jing Yang and Jian Du
Journal of Pharmacology and Experimental Therapeutics May 1, 2021, 377 (2) 254-264; DOI: https://doi.org/10.1124/jpet.120.000410
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Authorship Contributions:
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • SGS742 and Treatment of GHB Overdoses
  • Fate determination role of erythropoietin and romiplostim
  • Pharmacology of Antifentanyl mAb with Naloxone in Rats
Show more Drug Discovery and Translational Medicine

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics