Article Figures & Data
Figures
- Fig. 1.
Ang-(1–7) attenuated chronic IH–induced airway inflammation and collagen deposit in OVA-sensitized mice. (A) Representative images of H&E staining of lung tissue of the mice showed an increase in inflammatory cell infiltration in the OVA + chronic IH group compared with OVA group, which was attenuated by administration of Ang-(1–7) (150 µg/kg). Scale bar, 100 μm. (B) Representative Masson staining of lung tissues of the mice showed an increase in collagen deposit in the OVA + chronic IH group compared with OVA group, which was attenuated by administration of Ang-(1–7) (150 µg/kg). Scale bar, 100 μm. (C) Differential cell counts in BALF of the mice. (D) The levels of inflammatory factors IL-6 and IL-1β in BALF of the mice as measured using ELISA. Data are expressed as means ± S.D. (n = 6). *P: <0.05; **P < 0.01; ***P < 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test.
- Fig. 2.
Ang-(1–7) suppressed chronic IH–aggravated TGF-β–mediated EMT in OVA-sensitized mice. (A) Chronic IH decreased the level of epithelial marker E-cadherin and increased levels of mesenchymal markers α-SMA and collagen IV in airway epithelia of OVA-sensitized mice, which was reversed by administration of Ang-(1–7) (150 µg/kg). Representative images of immunofluorescence staining of lung tissues of the mice with antibodies against E-cadherin, α-SMA, and collagen IV were shown. Scale bar, 100 μm. (B) The average fluorescence intensity of E-cadherin, α-SMA, and collagen IV was shown. Data are expressed as means ± S.D. (n = 6). *P: <0.05; **P < 0.01; ***P < 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test. (C) Representative Western blots with antibodies against E-cadherin, α-SMA, and collagen IV in the indicated groups. β-Actin was used as the internal control. (D) Chronic IH increased the levels of TGF-β, p-Smad, and Snail in lung tissues of OVA-sensitized mice, which was reversed by administration of Ang-(1–7) (150 µg/kg). Representative Western blots with antibodies against TGF-β, p-Smad, Smad, and Snail were shown. β-Actin was used as the internal control.
- Fig. 3.
Ang-(1–7) suppressed IH-induced TGF-β–mediated EMT in LPS-treated human bronchial epithelial cells. (A) IH decreased the level of E-cadherin and increased levels of α-SMA and collagen IV in LPS (10 μg/ml)-treated human bronchial epithelial BEAS-2B cells, which was reversed by administration of Ang-(1–7) (100 nM) in a dose-dependent manner. Representative images of immunofluorescence staining of BEAS-2B cells with antibodies against E-cadherin, a-SMA, and collagen IV were shown. Scale bar, 20 μm. (B) The average fluorescence intensity of E-cadherin, α-SMA, and collagen IV was shown. Data are expressed as means ± S.D. (n = 6). *P: <0.05; **P < 0.01; ***P < 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test. (C) Representative Western blots with antibodies against E-cadherin, α-SMA, and collagen IV in the indicated groups. β-Actin was used as the internal control. (D) IH increased the levels of TGF-β, p-Smad, and Snail in LPS (10 μg/ml)-treated BEAS-2B cells, which was reversed by administration of Ang-(1–7) (100 nM) in a dose-dependent manner. Representative Western blots with antibodies against TGF-β, p-Smad, Smad, and Snail were shown. β-Actin was used as the internal control.
- Fig. 4.
A779 blocked the suppressive effects of Ang-(1–7) on TGF-β–mediated EMT upon IH exposure in LPS-treated human bronchial epithelial cells. (A) Representative images of immunofluorescence staining of BEAS-2B cells with antibodies against E-cadherin, α-SMA, and collagen IV were shown. Scale bar, 20 μm. (B) The average fluorescence intensity of E-cadherin, α-SMA, and collagen IV was shown. Data are expressed as means ± S.D. (n = 6). *P: <0.05; **P < 0.01; ***P < 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test. (C) Representative Western blots with antibodies against E-cadherin, α-SMA, and collagen IV in the indicated groups. β-Actin was used as the internal control. (D) Representative Western blots with antibodies against TGF-β, p-Smad, Smad, and Snail were shown. β-Actin was used as the internal control.
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Ang-(1–7) Reduces EMT in Asthma Combined with OSA
