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Research ArticleDrug Discovery and Translational Medicine
Open Access

Cell-Based No-Wash Fluorescence Assays for Compound Screens Using a Fluorescence Cytometry Plate Reader

Kirill Gorshkov, Manisha Pradhan, Miao Xu, Shu Yang, Emily M. Lee, Catherine Z. Chen, Min Shen and Wei Zheng
Journal of Pharmacology and Experimental Therapeutics September 2020, 374 (3) 500-511; DOI: https://doi.org/10.1124/jpet.120.265207
Kirill Gorshkov
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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  • ORCID record for Kirill Gorshkov
  • For correspondence: kirill.gorshkov@nih.gov
Manisha Pradhan
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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Miao Xu
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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Shu Yang
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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Emily M. Lee
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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Catherine Z. Chen
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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Min Shen
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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Wei Zheng
National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
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  • ORCID record for Wei Zheng
  • For correspondence: wzheng@mail.nih.gov
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Article Information

vol. 374 no. 3 500-511
DOI 
https://doi.org/10.1124/jpet.120.265207
PubMed 
32532853

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0022-3565
Online ISSN 
1521-0103
History 
  • Received February 3, 2020
  • Accepted June 1, 2020
  • Published online August 26, 2020.

Article Versions

  • Earlier version (June 12, 2020 - 11:16).
  • You are viewing the most recent version of this article.
Copyright & Usage 
U.S. Government work not protected by U.S. copyright

Author Information

  1. Kirill Gorshkov⇑,
  2. Manisha Pradhan,
  3. Miao Xu,
  4. Shu Yang,
  5. Emily M. Lee,
  6. Catherine Z. Chen,
  7. Min Shen, and
  8. Wei Zheng⇑
  1. National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland
  1. Address correspondence to:
    Dr. Kirill Gorshkov, NCATS/NIH, 9800 Medical Center Drive, MSC: 3375, Bethesda, MD 20892 E-mail: kirill.gorshkov{at}nih.gov; or
  2. Dr. Wei Zheng, NCATS/NIH, 9800 Medical Center Drive, MSC: 3375, Bethesda, MD 20892. E-mail: wzheng{at}mail.nih.gov
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Journal of Pharmacology and Experimental Therapeutics: 374 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 374, Issue 3
1 Sep 2020
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Research ArticleDrug Discovery and Translational Medicine

No-Wash Fluorescence Assays for Drug Screening

Kirill Gorshkov, Manisha Pradhan, Miao Xu, Shu Yang, Emily M. Lee, Catherine Z. Chen, Min Shen and Wei Zheng
Journal of Pharmacology and Experimental Therapeutics September 1, 2020, 374 (3) 500-511; DOI: https://doi.org/10.1124/jpet.120.265207

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Research ArticleDrug Discovery and Translational Medicine

No-Wash Fluorescence Assays for Drug Screening

Kirill Gorshkov, Manisha Pradhan, Miao Xu, Shu Yang, Emily M. Lee, Catherine Z. Chen, Min Shen and Wei Zheng
Journal of Pharmacology and Experimental Therapeutics September 1, 2020, 374 (3) 500-511; DOI: https://doi.org/10.1124/jpet.120.265207
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