Fig. 3. Upper panels are representative Western immunoblots of P‐eNOS (Ser‐1177), eNOS, P-Akt (Ser-473), Akt, P-nNOS (Ser-1412), nNOS, P-MYPT1, and MYPT1 in the bladder of WT + Empty-np, WT + NO-np, SCD + Empty-np, and SCD + NO-np. Lower panel represents quantitative analysis of P‐eNOS (Ser‐1177), eNOS, P-Akt (Ser-473), Akt, P-nNOS (Ser-1412), nNOS, P-MYPT1, and MYPT1 in the same treatment groups. Each bar represents the mean ± S.E.M. for 4–10 mice in each group. *P < 0.05 vs. WT, #P < 0.05 vs. Empty-np.