gRNA target 5′ N20-PAM -3′ | 5′ AGCGATCTGAACGCAAGAAG TGG 3′ |
ssODN template (reverse complement) | GTTTCGTCCTCGTGGAGCACCTGGTCATAGCCGCTGAGTGCGACACAGAAGATGATGGCCGTGACATCCTCAAAGCAGTGGATCCACTTCTTGtGTTCAGATCGCTGGCCCCCGACGTCAAACAGCCTGCAGGGAGTCAGGGAAAGCTGTGAGGGCGGGGACGCCTA |
PCR primers | O586 FWD: 5′ GGACAGGTGTCACAGGGGAT 3′ O587 REV: 5′ ACTGGCCTCCCTTGGCAATA 3′ |
Genotyping | By Sanger Sequencing |