Article Figures & Data
Figures
- Fig. 1.
High-salt diet increased systolic and diastolic blood pressure. Systolic (A) and diastolic (B) blood pressure in Dahl SS rats fed a high-salt diet (HS group) and low-salt diet (LS group) for 16 weeks measured by tail-cuff method. Data are expressed as mean ± S.E.M.; n = 7 per group. *P < 0.05 compared with LS group.
- Fig. 2.
Aortic remodeling in Dahl SS rats. Representative thoracic aorta cross-sections (A) stained with H&E. Medial cross-sectional area (B) was increased in the HS group. Wall-to-lumen ratio (C) was increased in the HS group. Data are expressed as mean ± S.E.M.; n = 8–12 per group. *P < 0.05 compared with LS group.
- Fig. 3.
T3 improved relaxation response in hypertensive aortas. Cumulative concentration curve and maximum response (Emax) to ACh in aortas from Dahl SS rats fed a high-salt diet (HS group) (A and B) and low-salt diet (LS group) (C and D). Aortas from both groups were pretreated with 0.1 μM T3 for 30 minutes (open squares and circles). Data are expressed as mean ± S.E.M.; n = 7 per group. **P < 0.05 compared with HS group.
- Fig. 4.
T3 attenuated augmented contractile response in hypertensive aortas. Cumulative concentration curve and maximum response (Emax) to PE in aortas from Dahl SS rats fed a high-salt diet (HS group) (A and B) and low-salt diet (LS group) (C and D). Aortas from both groups were pretreated with 0.1 μM T3 for 30 minutes (open squares and circles). Data are expressed as mean ± S.E.M.; n = 7 per group. **P < 0.05 compared with HS group.
- Fig. 5.
Decreased aortic PKG expression in Dahl SS rats fed a high-salt diet. Levels of PKG were assessed in aortas from the HS and LS groups using Western blot analysis. Top panel: representative immunoblots. Bottom panel: quantification of intensity of bands. Data are expressed as mean ± S.E.M., n = 7 per group, *P < 0.05 vs. LS group.
- Fig. 6.
T3 increased phosphorylation of VASP in hypertensive aortas. Aortas were treated with 0.1 µM T3 for 30 minutes. After treatment, levels of phosphorylated VASP in the serine 239 were assessed using Western blot analysis. Top panel: representative immunoblots. Bottom panel: quantification of intensity of bands. Data are expressed as mean ± S.E.M., n = 7 per group, *P < 0.05 vs. LS group, **P < 0.05 vs. HS group.
- Fig. 7.
T3 decreased oxidative stress in hypertensive aortas. Aortas from the experimental groups were either treated or not treated with 0.1 µM T3 for 30 minutes followed by detection of oxidative stress using DHE probe. Top panel: representative photomicrographs depicting DHE (red fluorescence) in aortas. Bottom panel: quantification of fluorescent intensity in each aorta cross-section. Data are expressed as mean ± S.E.M., n = 7 per group, *P < 0.05 vs. LS group, **P < 0.05 vs. HS group. Scale bar, 100 μm.
Additional Files
Data Supplement
- Supplemental Data -
Supplemental methods, 2 figures.
- Supplemental Data -
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