Article Figures & Data
Figures
- Fig. 1.
(A–C) Concentration-response curves to MK 571 (1–30 μM) in the isolated bladder (A), prostate (B), and urethra (C) from mice in tissues precontracted with carbachol (1 μM) or phenylephrine (10 μM). Data are the mean ± S.E.M. (n = 4 animals).
- Fig. 2.
Effect of MK 571 on relaxation responses induced by cAMP- and cGMP-stimulating substances in the isolated bladder. (A–D) Concentration-response curves to isoprenaline (0.001–100 μM; A), fenoterol (0.001–100 μM; B), forskolin (0.01–100 μM; C), and BAY 58-2667 (0.001–10 μM; D) in the absence (control) and presence of MK 571 (20 μM) in tissues precontracted with carbachol (1 μM). Data are the mean ± S.E.M. (n = 4 to 5 animals). *P < 0.05 compared with the control curves.
- Fig. 3.
Intracellular levels of cAMP and p-VASP Ser157 protein expression in the bladder. (A) Intracellular levels of cAMP were determined in unstimulated (basal) tissues and in tissues stimulated with fenoterol (3 μM) or forskolin (100 μM) in the absence and presence of MK 571 (20 μM). Data represent the mean ± S.E.M. (n = 4–6). *P < 0.05; #P < 0.05 compared with fenoterol/forskolin alone and basal, respectively. (B) Expression of p-VASP Ser157 and total VASP was determined in unstimulated (basal) tissues and tissues stimulated with fenoterol (3 μM) alone or in the presence of MK 571 (20 μM). Data are the mean ± S.E.M. (n = 5–7). **P < 0.001 compared with tissues stimulated with fenoterol. DMSO, dimethylsulfoxide.
- Fig. 4.
Effect of MK 571 on relaxation responses induced by cAMP- and cGMP-stimulating substances in the isolated prostate. (A–D) Concentration-response curves to sodium nitroprusside (0.001–100 μM; A), tadalafil (0.001–100 μM; B), 8-Br-cGMP (0.001–100 μM; C), and forskolin (0.001–100 μM; D) in the absence (control) and presence of MK 571 (20 μM) in tissues precontracted with phenylephrine (10 μM). Data are the mean ± S.E.M. (n = 4–7 animals). *P < 0.05; **P < 0.01; ***P < 0.001 compared with the control curves. SNP, sodium nitroprusside.
- Fig. 5.
Effect of MK 571 on relaxation responses induced by cAMP- and cGMP-stimulating substances in the isolated urethra. (A–D) Concentration-response curves to sodium nitroprusside (0.001–100 μM; A), BAY 41-2272 (0.001–1 μM; B), 8-Br-cGMP (0.001–1 μM; C), and forskolin (0.001–100 μM; D) in the absence (control) and presence of MK 571 (20 μM). Data are the mean ± S.E.M. (n = 4–6 animals). *P < 0.05 compared with the control curves. SNP, sodium nitroprusside.
- Fig. 6.
Intracellular levels of cGMP and p-VASP Ser239 protein expression in the prostate. (A) Intracellular levels of cGMP were determined in unstimulated (basal) tissues and in tissues stimulated with sodium nitroprusside (1 μM) or tadalafil (10 μM) in the absence and presence of MK 571 (20 μM). Data are the mean ± S.E.M. (n = 4–7). *P < 0.05 compared with sodium nitroprusside or tadalafil when added alone. (B) Protein expression of p-VASP Ser239 and total VASP was determined in unstimulated (basal) tissues and tissues stimulated with sodium nitroprusside (1 μM) alone or in the presence of MK 571 (20 μM). Data are the mean ± S.E.M. (n = 10–13). **P < 0.0001 compared with tissues stimulated with sodium nitroprusside; #P < 0.05 compared with basal levels. DMSO, dimethylsulfoxide; SNP, sodium nitroprusside.
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