Fig. 5. Indacaterol-induced genes in BEAS-2B cells. Probes sets meeting the expression criteria (>1.5-fold induction; FDR P value <0.1) were analyzed using Entrez Gene and the gene identification tool in DAVID to produce 134, 321, 205, and 9 upregulated genes at 1, 2, 6, and 18 hours, respectively. Induced genes that were subjected to independent PCR validation (Fig. 7) are in boldface and underlined. GO, NCBI Gene, and Gene Cards were used to manually assign each induced gene to one of six broad descriptors: 1) Transcriptional regulators, 2) Transporters, ion channels and membrane receptors, 3) Metabolic proteins, 4) General signaling molecules including translational regulators, 5) Other functions, and 6) Not assigned. Note: The following genes were detected with promiscuous probe sets (unannotated genes are not listed in the table): SIK1, LOC102724428 (208078_s_at, 232470_at), CHRNA7, LOC101929970, SLC24A2 (236385_at), AKIP1, NUAK2 (220987_s_at), SLC2A14, SLC2A3 (216236_s_at, 222088_s_at), HRK, LOC283454 (229552_at), ITGB6, LOC100505984 (208083_s_at), FAM87A, FAM87B (1559141_s_at, 1559140_at), CSNK1E, LOC400927-CSNK1E (222015_at, 226858_at), CREB5, LOC401317 (242329_at), RUNX1, LOC100506403 (210805_x_at) and UGT1A8, UGT1A9 (221305_s_at).