Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleDrug Discovery and Translational Medicine
Open Access

Altering Metabolic Profiles of Drugs by Precision Deuteration 2: Discovery of a Deuterated Analog of Ivacaftor with Differentiated Pharmacokinetics for Clinical Development

Scott L. Harbeson, Adam J. Morgan, Julie F. Liu, Ara M. Aslanian, Sophia Nguyen, Gary W. Bridson, Christopher L. Brummel, Lijun Wu, Roger D. Tung, Lana Pilja, Virginia Braman and Vinita Uttamsingh
Journal of Pharmacology and Experimental Therapeutics August 2017, 362 (2) 359-367; DOI: https://doi.org/10.1124/jpet.117.241497
Scott L. Harbeson
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adam J. Morgan
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie F. Liu
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ara M. Aslanian
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sophia Nguyen
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gary W. Bridson
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher L. Brummel
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lijun Wu
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Roger D. Tung
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lana Pilja
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Virginia Braman
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vinita Uttamsingh
Concert Pharmaceuticals, Inc., Lexington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF + SI
  • PDF
Loading

Abstract

Ivacaftor is currently used for the treatment of cystic fibrosis as both monotherapy (Kalydeco; Vertex Pharmaceuticals, Boston, MA) and combination therapy with lumacaftor (Orkambi; Vertex Pharmaceuticals). Each therapy targets specific patient populations: Kalydeco treats patients carrying one of nine gating mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) protein, whereas Orkambi treats patients homozygous for the F508del CFTR mutation. In this study, we explored the pharmacological and metabolic effects of precision deuteration chemistry on ivacaftor by synthesizing two novel deuterated ivacaftor analogs, CTP-656 (d9-ivacaftor) and d18-ivacaftor. Ivacaftor is administered twice daily and is extensively converted in humans to major metabolites M1 and M6; therefore, the corresponding deuterated metabolites were also prepared. Both CTP-656 and d18-ivacaftor showed in vitro pharmacologic potency similar to that in ivacaftor, and the deuterated M1 and M6 metabolites showed pharmacology equivalent to that in the corresponding metabolites of ivacaftor, which is consistent with the findings of previous studies of deuterated compounds. However, CTP-656 exhibited markedly enhanced stability when tested in vitro. The deuterium isotope effects for CTP-656 metabolism (DV = 3.8, DV/K = 2.2) were notably large for a cytochrome P450–mediated oxidation. The pharmacokinetic (PK) profile of CTP-656 and d18-ivacaftor were assessed in six healthy volunteers in a single-dose crossover study, which provided the basis for advancing CTP-656 in development. The overall PK profile, including the 15.9-hour half-life for CTP-656, suggests that CTP-656 may be dosed once daily, thereby enhancing patient adherence. Together, these data continue to validate deuterium substitution as a viable approach for creating novel therapeutic agents with properties potentially differentiated from existing drugs.

Introduction

Deuterium labeling of compounds has a long history of in vitro and in vivo use, primarily as a method to probe enzymatic mechanisms and metabolic pathways or to allow nonradioactive isotopic tracing (Rittenberg and Schoenheimer, 1937; Baillie, 1981). Deuterated agents as pharmaceuticals are an area of growing interest, as shown by the number of deuterated compounds entering clinical development (Halford, 2016). Additionally, the first deuterated compound, deutetrabenazine (Austedo, 2017), has recently been approved. A key feature of this technology is that deuterium substitution generally has minimal impact on the structure, physical properties, or pharmacology compared with the all-hydrogen version of a drug. However, deuterium substitution can have a marked effect upon the pharmacokinetics (PK) profile of a compound due to the deuterium isotope effect (DIE), which can lower the rate of carbon–deuterium versus carbon–hydrogen bond cleavage (Kushner et al., 1999; Nelson and Trager, 2003; Harbeson and Tung, 2011). Although the theoretical limit for the DIE is 6-fold to 9-fold, effects this large are rarely observed for enzyme-catalyzed reactions (Northrop, 1975; Jenks, 1987). The magnitude of the DIE for a cytochrome P450–mediated oxidation is unpredictable (Fisher et al., 2006; Harbeson and Tung, 2014). Due to this unpredictability, deuterium-substituted analogs must be synthesized and tested in preclinical studies to assess the magnitude and direction of the DIE. Even then, preclinical observations may not quantitatively, or potentially even qualitatively, predict human clinical results.

The first article in this series presented the data for CTP-347, a deuterated analog of paroxetine (Paxil; GlaxoSmithKline, Research Triangle Park, NC), which is a selective serotonin reuptake inhibitor for the treatment of a number of psychologic disorders (Paxil, 2012; Uttamsingh et al., 2015). Herein, we report the effects of selective deuterium substitution on another therapeutic agent, ivacaftor (Fig. 1). Ivacaftor (Kalydeco; Vertex Pharmaceuticals, Boston, MA) was approved in 2012 as the first drug to target specific mutational defects in the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) protein (Kalydeco, 2015). It is currently labeled for the treatment of CF patients 2 years and older who have at least one mutant allele from a group of nine gating mutations in the CFTR gene, with G551D being the most common (4.4% of CF patients in the United States) (Aditya, 2012; Kalydeco, 2015). In 2016, ivacaftor was approved as part of the fixed-dose combination drug Orkambi (Vertex Pharmaceuticals), which combines ivacaftor and lumacaftor for the treatment of CF patients 12 years and older homozygous for the F508del CFTR mutation (approximately 45% of CF patients in the United States) (Wainwright et al., 2015; Orkambi, 2016). Lumacaftor is a CFTR “corrector” that improves the formation of active CFTR at the epithelial surface of cells (Van Goor et al., 2011). The CFTR protein is an anion channel that regulates epithelial chloride and bicarbonate concentrations to maintain proper hydration and pH of secretory epithelia (Riordan, 2008). CFTR mutations reduce anion secretion, and the resulting dysregulation of anion concentrations promotes the accumulation of thick, viscous mucus. Ivacaftor is a CFTR “potentiator” and increases the channel open probability, thereby increasing anion conductance, improving mucus hydration and decreasing mucus viscosity (Van Goor et al., 2009). Viscous mucus is particularly problematic for lung function since it results in an inability to efficiently clear airways, which leads to chronic inflammation and infections that contribute to a decline in lung function over time (Ong and Ramsey, 2015).

Fig. 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 1.

Structures of CTP-656 (d9-ivacaftor), d18-ivacaftor, and ivacaftor. D, deuterium atom.

In this study, deuterated analogs of ivacaftor (Fig. 1) were prepared and characterized with respect to pharmacology, in vitro metabolism, and in vivo PK. Since ivacaftor is extensively metabolized in humans to two major circulating metabolites, M1 and M6, these metabolites and the corresponding deuterated metabolites d8-M1 and d6-M6 were also prepared and studied (Fig. 2) (Zha et al., 2011). Two compounds—d9-ivacaftor (CTP-656) and d18-ivacaftor—showed increased metabolic stability versus ivacaftor in vitro. Deuterium modification did not impact pharmacology, as the two deuterated compounds demonstrated equivalent potency to ivacaftor in multiple in vitro studies. In vitro metabolic stabilization did translate to increases in in vivo exposure parameters when the compounds were orally dosed in rats and dogs, although species differences were observed. Subsequently, both compounds were dosed in healthy volunteers in a single-dose crossover study. The new chemical entity CTP-656 was identified as the agent for further clinical advancement because of its general trend of increased exposure versus d18-ivacaftor. The observed increase in exposure and half-life (t1/2) compared with that reported for ivacaftor supported the potential for CTP-656 to be dosed once daily. These data continue to validate deuterium substitution as a viable approach to creating novel therapeutic agents with properties potentially differentiated from existing drugs.

Fig. 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 2.

CTP-656 and ivacaftor are metabolized in humans by CYP3A to two major metabolites.

Materials and Methods

Reagents

Indiplon, CTP-656, d18-ivacaftor, ivacaftor, M1, M6, d8-M1, and d6-M6 were provided by Concert Pharmaceuticals, Inc. (Lexington, MA). Human liver microsomes (HLM; mixed gender, pool of 200, 20 mg/ml) were from Xenotech, LLC (Lenexa, KS). Cryopreserved human hepatocytes were purchased from In Vitro ADMET Laboratories (Malden, MA). Human cDNA expressed CYP3A4 Supersomes (Corning, Corning, NY) were obtained from BD Biosciences (Woburn, MA). Dimethylsulfoxide (DMSO), acetonitrile, MgCl2, and NADPH were from Sigma-Aldrich (St. Louis, MO) and PEG400 was from J.T. Baker (Center Valley, PA). CF-HBE (human bronchial epithelial) cells were obtained from the Cystic Fibrosis Foundation Therapeutics organization. Forskolin was obtained from LC Laboratories (Woburn, MA). Benzamil and bumetanide were obtained from Sigma-Aldrich. HBE studies were conducted in the laboratory of Dr. Robert Bridges at Rosalind Franklin University of Medicine and Science, Chicago Medical School (North Chicago, IL).

Synthetic Procedures for the Synthesis of CTP-656, d18-Ivacaftor, d8-M1, d6-M6, M1, and M6

The synthetic methods for the preparation of CTP-656 and d18-ivacaftor have been previously described (Morgan, 2015). Analytical characterization of CTP-656 can be found in the Supplemental Material. These include liquid chromatography (LC) purity assessment (Supplemental Fig. 1), mass spectrum (Supplemental Fig. 2), and the 1H-NMR spectrum (Supplemental Fig. 3). Analytical data for d18-ivacaftor in the Supplemental Material include LC purity assessment (Supplemental Fig. 4), mass spectrum (Supplemental Fig. 5), and the 1H-NMR spectrum (Supplemental Fig. 6). Synthetic routes and methods for the preparation of CTP-656 metabolites d8-M1 and d6-M6 are also in the Supplemental Material (Supplemental Figs. 7 and 8, respectively). Analytical data for d8-M1 and d6-M6 in the Supplemental Material include LC purity assessments (Supplemental Fig. 9), mass spectra (Supplemental Fig. 10), and 1H-NMR spectra (Supplemental Fig. 11). The M1 metabolite of ivacaftor was prepared by a modification of a synthetic method previously described (Yang et al., 2010). The modified synthetic route (Supplemental Fig. 12) and the methods for the preparation of M1 can be found in the Supplemental Material. Ivacaftor metabolite M6 was prepared as previously described (Yang et al., 2010). Analytical data for M1 and M6 in the Supplemental Material include LC purity assessments (Supplemental Fig. 13), mass spectra (Supplemental Fig. 14), and 1H-NMR spectra (Supplemental Fig. 15).

Bioanalytical Methods

Preclinical Non–GLP Methods.

All plasma samples, in vitro samples, quality control, and standards were treated with acetonitrile during protein precipitation. Acetonitrile containing internal standard (IS; indiplon) was then added, and samples were vortexed at 1450 rpm for 3 minutes and then centrifuged at 3500 rpm for 10 minutes. Ten microliters of prepared sample was injected onto a high-performance LC (HPLC)–tandem quadrupole mass spectrometer (LC-MS/MS), consisting of an Agilent (Santa Clara, CA) 1200 HPLC spectrometer with a Leap HTS PAL auto-sampler (Leap Technologies, Morrisville, NC) equipped with a C6 Gemini phenyl 2.1 × 50 mm column maintained at 30°C. Analytes were eluted from the column using a gradient, where mobile phase B was linearly increased from 0% to 100% over 1 minute and then returned to 0% over another minute. The composition of mobile phase B was 0.1% formic acid in acetonitrile, whereas the composition of mobile phase A was 0.1% formic acid in reverse osmosis–deionized water.

LC-MS/MS analysis was performed using atmospheric pressure chemical ionization, followed by multiple reaction monitoring (MRM) of each analyte, including the IS. Transitions monitored were 393.2/172.2 m/z for ivacaftor, 402.2/172.2 m/z for CTP-656, 411.2/172.2 m/z for d18-ivacaftor, 417.2/172.2 m/z for d8-M1, and 409.2/172.2 m/z for M1, whereas the transition for indiplon (the IS) was 377.2/293.2 m/z. For all analytes, a dwell time of 100 milliseconds was used.

Clinical GLP Method.

The determination of CTP-656 and d18-ivacaftor in plasma was performed using a validated method under by CPR Pharma Services (Thebarton, SA, Australia) conducted in compliance with CPR Pharma Standard Operation Procedures. Plasma analysis was performed after protein precipitation with 99% acetonitrile and 1% formic acid using ivacaftor as the IS. The analytes were separated by HPLC using a C18 column (50 × 2 mm, 3 µm; Luna C18; Phenomenex, Torrance, CA), where mobile phase A consisted of 10% acetonitrile and 90% water with 0.1% formic acid and mobile phase B consisted of 50% acetonitrile, 50% methanol with 0.1% formic acid. Elution of the analytes from the column was performed at a flow rate of 0.3 ml/min using a gradient where the percentage of mobile phase B was increased linearly from 55% to 100% over 2.5 minutes and held at 100% for 1 minute, after which the mobile phase composition was set to 10% mobile phase B for 0.4 minutes and then returned to initial conditions (55% mobile phase B) for re-equilibration. The total run time for the method was 6 minutes.

The analysis of the eluates was performed using a mass spectrometry system (API 4000 System; AB Sciex, Framingham, MA) in positive MRM mode. The MRM transitions monitored were 402.2/172.2 m/z for CTP-656 and 411.2/172.2 m/z for d18-ivacaftor. Ivacaftor was the IS for both CTP-656 and d18-ivacaftor. For all analytes, the method was linear, and in all cases the precision and accuracy met the criteria of the Food and Drug Administration and the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use.

In Vitro Pharmacology Assessments

Chloride conductance was evaluated with an equivalent current (Ieq) assay system using fully differentiated primary cultures of CF-HBE cells grown at an air/liquid interface for 4–6 weeks. Transepithelial voltage and transepithelial conductance were measured under current-clamp conditions using a custom-designed 24-channel current clamp (TECC-24; ChanTest Corporation, Cleveland, OH) and a 24-well electrode manifold. For all experiments, cells were tested in symmetric chloride solution (HEPES-buffered Coon’s F-12 modified liquid media without serum or bicarbonate) at 36 ± 0.5°C, and all compounds and test articles were dissolved in DMSO. For chloride conductance measurements, epithelial sodium channels were blocked with 3 µM benzamil; potentiator responses were measured after 10 µM forskolin addition. CFTR specificity was determined by blocking chloride secretion with 20 µM bumetanide. Potentiator responses were quantified by calculating the total peak area under the curve (AUC) of Ieq after addition of test article. For studies using a corrector, lumacaftor was added at a concentration of 3 µM 24 hours prior to chloride conductance measurements. All compounds were assayed in triplicate at a single concentration per well, and AUC values were fit to a Hill equation to yield EC50 values.

Metabolic Stability in CYP3A4 Supersomes

Stock solutions (10 mM) of CTP-656, d18-ivacaftor, and ivacaftor were prepared in DMSO. The stock solutions were diluted to 50 µM in acetonitrile. Human CYP3A4 Supersomes (1000 pmol/ml) were diluted to 62.5 pmol/ml in 0.1 M potassium phosphate buffer, pH 7.4, containing 3 mM MgCl2. Metabolic stability in the diluted Supersomes was assessed as previously described (Morgan, 2015).

Semiquantitative Metabolite Profile in Human Hepatocytes

Stock solutions (5 mM) of CTP-656, d18-ivacaftor, and ivacaftor were prepared in DMSO. Cryopreserved human hepatocytes were thawed, diluted in cold thawing media, and centrifuged. The supernatants were removed, and the cells were resuspended in Krebs-Henseleit buffer. The test articles were added to the hepatocytes in Krebs-Henseleit buffer to achieve a final concentration of 25 µM in 0.5 ml, containing 4 × 106 cells/ml hepatocytes. The reaction mixtures were incubated at 37°C in a cell culture incubator (5% CO2). Aliquots of the mixture were removed at 0 and 4 hours, and acetonitrile was added. Samples were transferred to microcentrifuge tubes and centrifuged at 10,000g for 8 minutes to pellet the precipitated proteins. Supernatants were analyzed for amounts of the parent remaining and metabolites formed by LC-MS/MS. UV-visible light at 254 nm was used for semiquantitative analysis, wherein metabolites were measured as percentage of parent peak area. Analysis was by UV-visible light since metabolite standards had not been synthesized at the time of this experiment.

Determination of DIE for CTP-656 in HLM

Stock solutions (50 mM) of test compounds were prepared in DMSO. The 20 mg/ml HLM were diluted to 0.625 mg/ml in 0.1 M potassium phosphate buffer, pH 7.4, containing 3 mM MgCl2. Aliquots (375 µl) of the HLM and test compound mixtures were transferred to wells of a deep 96-well plate in triplicate and prewarmed for 7 minutes. Reactions were initiated by the addition of 125 µl of prewarmed NADPH solution. The final reaction volume was 0.5 ml and contained 0.5 mg/ml HLM; 1, 2, 5, 10, 20, 40, 60, 80, and 100 µM ivacaftor or CTP-656; 2 mM NADPH; and 3 mM MgCl2. The reaction mixtures were incubated at 37°C for 10 minutes, after which 50 µl aliquots were removed and added to shallow 96-well plates. The reactions were stopped by the addition of 50 µl of ice-cold acetonitrile with IS. The plates were stored at 4°C for 20 minutes, after which 100 µl of water was added to the wells before centrifugation to pellet precipitated proteins. Supernatants were transferred to another 96-well plate and analyzed for parent and metabolite by LC-MS/MS using an AB Sciex API 4000 mass spectrometer.

The kinetic parameters Km (concentration of substrate at one-half Vmax) and Vmax (maximum velocity) were calculated from the Michaelis-Menten equation. The enzyme velocity (enzyme velocity = concentration of hydroxyl methyl/time/protein concentration) from three separate experiments was analyzed by GraphPad Prism version 6.04 (GraphPad Software, San Diego, CA), and reported values for Km and Vmax were determined from the fit using the average value.

Preclinical PK Studies

All animal studies were carried out in accordance with the Guide for the Care and Use of Laboratory Animals, as adopted and promulgated by the US National Institutes of Health, and were approved by the National Institutes of Health Animal Care and Use Committee or the local equivalent.

PK of CTP-656, d18-Ivacaftor and Ivacaftor in Rats.

Male Sprague-Dawley rats (234-276 g) were obtained from Harlan Laboratories (Indianapolis, IN) and housed at VivoPath, LLC (Worcester, MA), where the studies were conducted. The PK of CTP-656, d18-ivacaftor, and ivacaftor in rats were assessed as previously described (Morgan, 2015). The plasma PK parameters for the dosed compounds were calculated by noncompartmental analysis using Phoenix 64 WinNonlin software (Certara, Princeton, NJ).

PK of CTP-656, d18-Ivacaftor, and Ivacaftor in Dogs.

Four male beagle dogs were housed at Agilux Laboratories (Worcester, MA), where the studies were conducted. The dogs were administered 3 mg/kg each test compound orally in a discrete-dose crossover study with a 1-week washout period between the dosing phases. All dogs were fasted overnight before the administration of each test article. Each compound was formulated as a solution in 100% PEG400 at a concentration of 1.5 mg/ml. Blood samples were collected from each dog at 15 and 30 minutes, and at 1, 2, 4, 6, 8, 16, 24, 48, 72, 84, and 96 hours postdose. Blood samples were centrifuged to obtain plasma samples that were analyzed for concentrations of the dosed compound at each time point using LC-MS/MS. The limit of quantitation of each compound was 1 ng/ml. The plasma PK parameters for the dosed compounds were calculated by noncompartmental analysis using Phoenix 64 WinNonlin software (Certara).

Evaluation of PK Profiles of CTP-656 and d18-Ivacaftor in Healthy Human Volunteers

The first-in-human study was a two-part open-label, randomized, sequential, crossover, single-dose study to evaluate the safety, tolerability, and PK of CTP-656 and d18-ivacaftor amorphous dispersion aqueous suspension in healthy volunteers, with a PK comparison with ivacaftor. This article will report on the first part of this clinical study, which was a single-dose, randomized, two-period, two-treatment, crossover study conducted in healthy male and female volunteers to evaluate the safety, tolerability, and PK of CTP-656 and d18-ivacaftor under fasted conditions. The study was performed at a single center [CMAX (a division of IDT Australia Limited), Adelaide, SA, Australia] under the Clinical Trial Notification scheme through the Therapeutic Goods Administration. The study was conducted in accordance with the Declaration of Helsinki (e.g., the clinical study protocol, amendments, and informed consent documents), and any other study-related documents were reviewed and approved by the Bellberry Human Research Ethics Committee. Informed consent was obtained prior to any study-related procedures.

Six healthy volunteers were enrolled in this part of the study. On Day 1, three subjects received a single oral dose of 25 mg of CTP-656 and three subjects received a single oral dose of 25 mg of d18-ivacaftor as an aqueous suspension. On Day 8 after the first dose, subjects crossed over and the dosing was repeated. A total of 21 PK blood samples (4 ml each) were collected for the assessment of plasma concentrations of CTP-656 and d18-ivacaftor. Time points for collections were immediately prior to dosing (0 hour), and 0.25, 0.5, 1, 2, 3, 4, 5, 6, 8, 10, 12, 16, 20, 24, 36, 48, 60, 72, 84, and 96 hours postdose. Plasma levels of CTP-656 and d18-ivacaftor were measured by LC-MS/MS. The plasma PK parameters for the dosed compounds were calculated by noncompartmental analysis using Phoenix 64 WinNonlin software (Certara). Each subject was monitored for safety throughout the study, including obtaining vital signs, performing physical examinations, obtaining clinical laboratory measurements, and performing 12-lead electrocardiograms.

Results

Deuterium Substitution Does Not Alter the Pharmacology of Ivacaftor and Its Major Metabolites.

The pharmacological properties of CTP-656 and its major metabolites, d8-M1 and d6-M6, were compared with ivacaftor, M1, and M6 using a chloride conductance assay with fully differentiated HBE cells expressing mutant CFTR. It has been reported for the G551D CFTR mutation that M1 has approximately one-sixth the potency and M6 less than 1/50 the potency of ivacaftor (Kalydeco, 2015). In G551D/F508del HBE cells, ivacaftor and CTP-656 had similar potencies for potentiating forskolin responses, with EC50 values of 336 and 255 nM, respectively (Table 1). The potencies of the M1 and d8-M1 metabolites were also similar (0.96 and 1.35 µM, respectively), and both metabolites were less potent than the parent: 3-fold for M1 and 5-fold for d8-M1. The EC50 values for M6 and d6-M6 were 11 and 15 µM, respectively, which is in agreement with prior reports that the M6 metabolite does not contribute to the therapeutic efficacy of ivacaftor in CF patients (Kalydeco, 2015).

View this table:
  • View inline
  • View popup
TABLE 1

Potency of CFTR potentiation in G551D/F508del HBE cells by ivacaftor and deuterated ivacaftor analogs

To compare the activities of CTP-656, ivacaftor, and their metabolites in the context of pharmacologically corrected F508del-CFTR, the potencies of all compounds for potentiating forskolin responses were tested in F508del/F508del HBE cells after a 24-hour incubation period with lumacaftor. Similar to results obtained with G551D/F508del HBE, the potencies of nondeuterated and deuterated parent and metabolite compounds were very similar (Table 2). d18-Ivacaftor was also tested in G551D-CFTR–expressing FRT (Fischer rat thyroid) cells and lumacaftor-corrected F508del/F508del HBE cells; no significant differences in potentiation activity or potency were observed among d18-ivacaftor, CTP-656, and ivacaftor (data not shown).

View this table:
  • View inline
  • View popup
TABLE 2

Potency of CFTR potentiation in lumacaftor-corrected F508del/F508del HBE cells by ivacaftor and deuterated ivacaftor analogs

Deuterated Ivacaftor Analogs Show Increased Metabolic Stability in In Vitro Assays.

Metabolic stability was initially determined by incubating each compound in the presence of CYP3A4 Supersomes for 30 minutes and monitoring the decrease in compound versus time. Additionally, compounds were incubated with human hepatocytes, and the amounts of parent remaining and metabolites formed were measured. In CYP3A4 Supersomes, the in vitro t1/2 value for ivacaftor was 5.5 minutes, whereas the t1/2 values for CTP-656 and d18-ivacaftor were both 8.1 minutes (average of two experiments for the three compounds tested), which is a 47% increase in t1/2 for CTP-656 and d18-ivacaftor versus ivacaftor (Fig. 3A). The data from human hepatocytes showed an approximate 2-fold reduction of the M1 metabolites as a percentage of parent for both CTP-656 and d18-ivacaftor with respect to ivacaftor (Table 3). Additionally, no new metabolites were identified in the human hepatocyte incubations of the two deuterium-modified compounds.

Fig. 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 3.

In vitro metabolism. (A) t1/2 values of ivacaftor and d-ivacaftor analogs in human CYP3A4 Supersomes. (B) Deuterium kinetic isotope effect as measured by the rate of formation of M1 and d8-M1 metabolites in HLM.

View this table:
  • View inline
  • View popup
TABLE 3

Semiquantitive analysis of M1 metabolites versus parent after incubation of ivacaftor, CTP-656, and d18-ivacaftor in human hepatocytes

Deuterium-enhanced metabolic stability was also assessed by incubating increasing concentrations of ivacaftor and CTP-656 in the presence of HLM for 10 minutes and measuring the rate of formation for M1 and d8-M1, respectively. The data were then fit to the hyperbolic form of the Michaelis-Menten equation to derive the kinetic parameters Km, Vmax (enzyme velocity at saturating substrate: [S] > > Km), and Vmax/Km (enzyme velocity at low substrate concentrations: [S] < < Km). The ratios of the kinetic parameters Vmax and Vmax/Km for ivacaftor versus CTP-656 measure the deuterium kinetic isotope effects DV (VmaxH/VmaxD) and DV/K [(Vmax/Km)H/(Vmax/Km)D] for the CYP3A4-catalyzed formation of M1 in HLM (Northrop, 1975). Significant DIEs were measured for both kinetic parameters: DV = 3.8 and DV/K = 2.2 (Fig. 3B). The observed increase in t1/2 for CTP-656 and d18-ivacaftor versus ivacaftor in CYP3A4 Supersomes, the decreased metabolism in human hepatocytes, and the isotope effects observed in HLMs supported progression of the compounds to in vivo PK studies in rats and dogs.

Deuterated Ivacaftor Analogs Show Differentiated Nonclinical PK Upon Oral Dosing.

The PK profiles of the three compounds were initially assessed in male Sprague-Dawley rats. Each compound was dosed at 10 mg/kg to each of three rats, with a total of nine rats in the study. The plasma levels of compound were measured over 72 hours. The plasma concentration versus time curves for drug exposure of the compounds are shown in Fig. 4A (only the 0 to 24-hour data are shown for clarity). Although d18-ivacaftor showed greater exposure than either CTP-656 or ivacaftor, the error bars for the time points are overlapping, indicating modest differentiation for d18-ivacaftor versus ivacaftor. This modest differentiation is supported by the PK parameters for the three compounds (Table 4). Across the parameters Cmax, AUC0–24 hour, C24 hour and t1/2, d18-ivacaftor showed a trend to larger exposures in rats compared with both CTP-656 and ivacaftor.

Fig. 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 4.

In vivo metabolism. (A) Exposure profiles in male Sprague-Dawley rats (n = 3/group) after oral dosing of CTP-656, d18-ivacaftor, and ivacaftor. (B) Exposure profiles in male beagle dogs (n = 4) after oral dosing of CTP-656, d18-ivacaftor, and ivacaftor in a crossover study.

View this table:
  • View inline
  • View popup
TABLE 4.

Mean PK parameters (%CV) for CTP-656, d18-ivacaftor, and ivacaftor in male Sprague-Dawley rats (10 mg/kg) and male beagle dogs (3.0 mg/kg) after oral administration

The PK profiles of the three compounds were also studied in male beagle dogs. Each compound was dosed by oral gavage at 3 mg/kg to each of four dogs in a crossover fashion with a 1-week washout period between doses, and the plasma levels of compound were measured over 96 hours. As shown by the plasma concentration versus time profiles for each compound (Fig. 4B), CTP-656 had much greater exposure than ivacaftor and appeared to exhibit greater exposure than d18-ivacaftor (only the 0 to 24-hour data are shown for clarity). The PK parameters for CTP-656 versus ivacaftor also supported the differentiated profile versus ivacaftor in dogs (Table 4). The Cmax, AUC0–24hr, and C24hour values show 62%, 69%, and 83% increases, respectively, versus ivacaftor.

Although the PK data for CTP-656 and d18-ivacaftor in rats and dogs demonstrated deuterium-mediated slowing of ivacaftor metabolism, the data did not clearly identify which compound should be advanced to human studies. The Pharmacology Review section of the Summary Basis of Approval for ivacaftor noted that exposure to ivacaftor was significantly greater than M1 and M6 in nonclinical test species (mouse, rat, and dog) (Center for Drug Evaluation and Research, Pharmacology Review(s): Ivacaftor (Application Number: 203188Orig1s000); http://www.accessdata.fda.gov/drugsatfda_docs/nda/2012/203188Orig1s000PharmR.pdf). However, in humans, the exposure to M1 and M6 is significantly greater than ivacaftor. In view of the extensive metabolism of ivacaftor that occurs in humans, but is not observed in either rats or dogs, both CTP-656 and d18-ivacaftor were advanced to a first-in-human study to identify the preferred compound for continued clinical development.

CTP-656 Identified as the Deuterated Ivacaftor Analog for Clinical Advancement.

This first-in-human study of CTP-656 and d18-ivacaftor was designed as a two-part crossover study of a low (25 mg) single oral dose of the two compounds as an aqueous suspension of their respective solid, amorphous dispersions. The baseline demographics of the study participants are summarized in Table 5. In the first part, six healthy volunteers (4 male and 2 female) were administered study drug on Day 1: three received CTP-656 and three received d18-ivacaftor. On Day 8, the subjects were crossed over and were administered the other compound. Blood samples were collected over a 96-hour period on Days 1 and 8, and the PK parameters were determined (Table 6). The plasma concentration versus time curves (0–24 hours shown for clarity) for each compound are also shown (Fig. 5).

View this table:
  • View inline
  • View popup
TABLE 5

Subject demographics

View this table:
  • View inline
  • View popup
TABLE 6

Mean PK parameters (%CV) for CTP-656 and d18-ivacaftor in healthy males and females after oral administration of a single 25-mg dose

Fig. 5.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 5.

Exposure profiles in healthy volunteers (n = 6) after oral dosing of CTP-656 and d18-ivacaftor.

The PK parameters reflect the general trend shown in the exposure curves in that Cmax and AUC from time 0 extrapolated to infinity (AUC0–inf) values are greater for CTP-656 than for d18-ivacaftor. The approximate 16-hour t1/2 of CTP-656 is also compatible with identifying a dose that would enable once-daily dosing. PK data for a 25 mg dose of ivacaftor have been previously reported as part of a single–ascending dose (SAD) study (Center for Drug Evaluation and Research, Clinical Pharmacology and Biopharmaceutics Review(s): Ivacaftor (Application Number: 203188Orig1s000); http://www.accessdata.fda.gov/drugsatfda_docs/nda/2012/203188Orig1s000ClinPharmR.pdf). For comparative purposes, the t1/2 for ivacaftor was 11.1 hours, and the exposure (AUC0–inf) was 1627 h*ng/mL. These data for ivacaftor suggest that at the same dose CTP-656 may have greater exposure and a longer t1/2 versus ivacaftor in humans. In subsequent clinical studies, CTP-656 as a solid oral 150 mg dose was compared directly to a single dose of ivacaftor (150 mg tablet) and was shown to have an approximate 15-hour t1/2 (Uttamsingh et al., 2016).

Discussion

This study reports the results of precision deuteration of ivacaftor (Kalydeco; Vertex Pharmaceuticals), which is the first approved CFTR modulator for the treatment of CF. Two deuterated analogs, d9-ivacaftor (CTP-656) and d18-ivacaftor were tested in vitro and in vivo to assess the pharmacology, PK, and metabolism of the compounds with respect to ivacaftor. The studies of the deuterated ivacaftor analogs were designed to assess whether a DIE could reduce metabolism in humans and provide an agent with a differentiated clinical profile. The data for the deuterated analogs of ivacaftor align with the previous article in this series (Uttamsingh et al., 2015) that assessed the effects of precision deuteration on paroxetine pharmacology and PK. Deuterated paroxetine (CTP-347) retained the intrinsic in vitro pharmacology profile of paroxetine with greatly reduced mechanism-based inactivation of CYP2D6 in in vitro studies. This preclinical profile of CTP-347 translated to a reduced drug-drug interaction liability with dextromethorphan in a clinical study. However, further clinical development of CTP-347 as a nonhormonal therapy for hot flashes was stopped for commercial reasons.

To confirm that deuterium substitution did not affect the intrinsic pharmacology of ivacaftor, in vitro studies were performed in HBE cells derived from CF patients. The potency as a potentiator of CFTR with a gating mutation was measured in G551D/F508del HBE cells. For homozygous F508del HBE cells, the cells were first corrected with lumacaftor prior to measuring the potentiation of conductance. Ivacaftor is extensively metabolized in humans to two major circulating metabolites, M1 and M6 (Zha et al., 2011). At steady state, the metabolites are the major circulating species in humans, with mean exposure ratios (metabolite/parent) of approximately 5-fold and 2-fold, respectively, for M1 and M6 (Center for Drug Evaluation and Research, Clinical Pharmacology and Biopharmaceutics Review(s): Ivacaftor (Application Number: 203188Orig1s000); http://www.accessdata.fda.gov/drugsatfda_docs/nda/2012/203188Orig1s000ClinPharmR.pdf). These metabolites of ivacaftor and CTP-656 were, therefore, also evaluated in the HBE cells assays.

Overall, the in vitro pharmacology data confirmed that deuteration did not change the CFTR activity of ivacaftor and the major metabolites. The equivalent activity of CTP-656 and ivacaftor measured in G551D/F508del HBE cells supports the use of CTP-656 in the same patient population as ivacaftor. Likewise, the potentiation of CFTR function in lumacaftor-corrected F508del homozygous HBE cells supports the clinical use of CTP-656 in combination with lumacaftor and potentially other correctors currently in development.

Although precision deuteration of ivacaftor did not change the intrinsic pharmacology of the compound, decreases in metabolism for the deuterated analogs were shown both in vitro and in vivo. High protein binding, nonspecific binding, and low solubility prevented the metabolic stability assessment of ivacaftor in our standard HLM assay due to the slow apparent rate of metabolism (unpublished data). Since ivacaftor is metabolized primarily by CYP3A4 (Kalydeco, 2015), CYP3A4 Supersomes were used to assess metabolic stability. The two deuterated analogs exhibited t1/2 values approximately 50% greater than ivacaftor in this study, which signaled that precision deuteration could positively impact the PK of ivacaftor.

Once the respective M1 and d8-M1 metabolites of ivacaftor and CTP-656 were synthesized, a more sensitive assay to measure metabolism in HLM was possible. By measuring the formation of metabolites instead of the loss of parent, a direct measurement of metabolite formation in HLM was possible and enabled calculation of the DIE using classic Michaelis-Menten kinetics. The measured DV/K and DV values for CYP3A4 catalyzed metabolism in HLM were large.

Although a DV/K isotope effect of 2.2 was measured, the literature reports that an isotope effect on V/K should not be observed unless it has been unmasked, which can occur under certain conditions (Korzekwa et al., 1989; Nelson and Trager, 2003). Unmasking could arise due to oxidation at a different site on CTP-656 to produce an alternate metabolite (“metabolic switching”) (Horning et al., 1975). Preclinical and clinical data, however, do not suggest metabolic switching. Incubations of CTP-656 and d18-ivacaftor in human hepatocytes showed only reductions in the formation of the M1 metabolites versus ivacaftor; no other metabolites were detected. Clinical data also showed the same plasma metabolites for CTP-656 and ivacaftor in humans (manuscript in preparation). Another possible mechanism for unmasking the isotope effect is direct reduction of the activated enzyme-substrate complex to produce free enzyme, substrate, and water (Atkins and Sligar, 1987). Studies were not conducted to assess this mechanism, but it appears to be a reasonable explanation for the observed DV/K isotope effect for CTP-656 in the absence of metabolic switching.

A recent review (Guengerich, 2013) on the potential issues for deuterium in drug development notes than an isotope effect in vitro needs to translate to a PK effect in vivo to have a practical application to drug development. Although slower metabolism was observed in vitro, the translation to in vivo is unpredictable since non–cytochrome P450 clearance mechanisms may predominate in vivo and clearance mechanisms may vary between species. PK studies in rats and dogs were particularly challenging since neither rats nor dogs metabolize ivacaftor as extensively as humans; therefore, these preclinical species may not be adequate models for human metabolism (Center for Drug Evaluation and Research; Pharmacology Review(s): Ivacaftor (Application Number: 203188Orig1s000); http://www.accessdata.fda.gov/drugsatfda_docs/nda/2012/203188Orig1s000PharmR.pdf). The PK results in rats and dogs for CTP-656 were encouraging, with a longer t1/2 and greater exposure versus ivacaftor in both species. d18-Ivacaftor, however, showed increased exposure versus ivacaftor in rats, but not in dogs. These results emphasize the unpredictable effects of deuterium substitution. Although positive preclinical PK effects were very encouraging, these models were not sufficient to choose a preferred compound for clinical development. The more rigorous approach involved testing both compounds in humans and selecting a compound for advancement based upon human PK data.

The clinical crossover study of orally dosed CTP-656 and d18-ivacaftor identified the preferred compound for further clinical development. CTP-656 exposure was only modestly increased versus d18-ivacaftor; however, the increases were consistent across the PK parameters Cmax, t1/2, AUC0–inf, C24hr and oral clearance (CL/F). These data, coupled with the reduced amount of deuterium in CTP-656, were sufficient to identify CTP-656 for continued clinical development.

The potential of CTP-656 to be dosed once daily was predicted based on available information for ivacaftor, including SAD PK in healthy volunteers and PK/PD relationships in patients (Center for Drug Evaluation and Research, Clinical Pharmacology and Biopharmaceutics Review(s): Ivacaftor (Application Number: 203188Orig1s000); http://www.accessdata.fda.gov/drugsatfda_docs/nda/2012/203188Orig1s000ClinPharmR.pdf). In the SAD study of ivacaftor, which was conducted in the fasted condition, exposure increases were dose proportional from 25 to 375 mg. Additionally, the 150 mg twice-daily dose of ivacaftor was selected based on simulations that showed a plasma Cmin at steady state of ∼250 ng/ml achieved the predicted EC90 for forced expiratory volume in 1 s and the EC84 for sweat chloride. The 25 mg dose of CTP-656 in the fasted state provided a C24hr (Cmin) of 53 ng/ml in plasma. Assuming dose-proportional increases in exposure for CTP-656, a once-daily dose of approximately 118 mg in the fasted condition would achieve the target C24hr of 250 ng/ml even in the absence of accumulation on multiple dosing. Ivacaftor shows an increase in exposure of 2-fold to 3-fold when dosed with food; therefore, CTP-656 has the potential to exceed the 250 ng/ml Cmin at steady state if a similar food effect is observed. Based on this analysis of the 25 mg dose, CTP-656 was projected to achieve the desired once-daily profile in future studies, which was confirmed in subsequent phase I studies.

Once-daily dosing may translate to improved adherence in real-world use. A small clinical study (Siracusa et al., 2015) assessed ivacaftor adherence in patients and found the adherence to be highly variable. The ivacaftor label (Kalydeco, 2015) specifies dosing every 12 hours; however, this study found that the median duration between doses was 16.9 hours, with a range of 12.1–43.7 hours. The overall adherence rate—calculated as (total doses taken)/(total days monitored × 2 doses/d)—was only 61%. It is also noteworthy that the 10.4% improvement in absolute percent predicted forced expiratory volume in 1 s observed in the clinical study (≥12 years old) is much greater than the 5.4% improvement observed over 12 months in a non–clinical trial patient population (Fink et al., 2015). Although this may be due to a number of factors, the suboptimal adherence could be an important contributing factor, which may be positively impacted by a once-daily potentiator.

CTP-656 has completed three phase I studies in healthy volunteers, including SAD, multiple–ascending dose, and food-effect studies. The tablet formulation was reported to have an average t1/2 of 15 hours after a single oral dose and an average t1/2 of 18 hours in the multiple–ascending dose studies (Uttamsingh et al., 2016). Of particular note is the reported CTP-656/d8-M1 AUC ratio of 1.5:1 after a single dose and also at steady state. This is much greater than ivacaftor, which has a parent/M1 AUC ratio of 1:5 at steady state. With a longer t1/2 and greater exposure to the more active parent, the PK profile for CTP-656 may translate into an improved clinical profile in CF patients with gating mutations. A phase II safety and efficacy study in CF patients is currently underway (NCT02971839).

Acknowledgments

We thank Doug Williams, Kinga Chojnacka, and Junfeng Huang (Adesis Inc., New Castle, DE) for metabolite synthesis; and David Norris (Ecosse Medical Communications, Falmouth, MA) for editorial assistance.

Authorship Contributions

Participated in research design: Harbeson, Liu, Aslanian, Brummel, Wu, Tung, Braman, and Uttamsingh.

Conducted experiments: Morgan, Aslanian, Nguyen, Bridson, Pilja, and Uttamsingh.

Contributed new reagents or analytic tools: Morgan, Liu, Bridson, and Brummel.

Performed data analysis: Aslanian, Nguyen, Brummel, and Uttamsingh.

Wrote or contributed to the writing of the manuscript: Harbeson, Liu, Aslanian, Brummel, Tung, Braman, and Uttamsingh.

Footnotes

    • Received March 25, 2017.
    • Accepted June 8, 2017.
  • This research was supported by Concert Pharmaceuticals, Inc., Lexington, Massachusetts. L.W. and L.P. are former employees of Concert Pharmaceuticals, Inc.

  • https://doi.org/10.1124/jpet.117.241497.

  • ↵Embedded ImageThis article has supplemental material available at jpet.aspetjournals.org.

Abbreviations

AUC
area under the curve
AUC0–24hr
area under the curve from time 0 to 24 hours postdose
AUC0–inf
area under the curve from time 0 extrapolated to infinity
C24hr
concentration at 24 hours postdose
Cmax
maximum plasma concentration
CF
cystic fibrosis
CFTR
cystic fibrosis transmembrane conductance regulator
CTP-656
N-(2-(tert-butyl)-4-(tert-butyl-d9)-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide
DIE
deuterium isotope effect
DMSO
dimethylsulfoxide
GLP
good laboratory practice
HBE
human bronchial epithelial
HLM
human liver microsomes
HPLC
high-performance liquid chromatography
Ieq
equivalent current
IS
internal standard
Km
concentration of substrate at one-half Vmax
LC
liquid chromatography
LC-MS/MS
liquid chromatography-tandem mass spectrometry
MRM
multiple reaction monitoring
PK
pharmacokinetics
SAD
single ascending dose
t1/2
half-life
  • Copyright © 2017 by The Author(s)

This is an open access article distributed under the CC BY Attribution 4.0 International license.

References

  1. ↵
    1. Aditya S
    (2012) Ivacaftor in cystic fibrosis: the first disease modifying agent. Int J Basic Clin Pharmacol 1:225–229.
    OpenUrl
  2. ↵
    1. Atkins WM and
    2. Sligar SG
    (1987) Metabolic switching in cyctochrome P-450cam: deuterium isotope effects on regiospecificity and the monooxygenase/oxidase ratio. J Am Chem Soc 109:3754–3760.
    OpenUrlCrossRef
  3. ↵
    Austedo. (2017) Package insert. Teva Pharmaceuticals USA, North Wales, PA.
  4. ↵
    1. Baillie TA
    (1981) The use of stable isotopes in pharmacological research. Pharmacol Rev 33:81–132.
    OpenUrlPubMed
  5. ↵
    Fink A, Sawicki GS, Morgan WJ, Schechter MS, Rosenfeld M, and Marshall BC (2015) Treatment response to ivacaftor in clinical practice: analysis of the US CF Foundation patient registry. Pediatr Pulmonol 50:361 (Abstract 450).
  6. ↵
    1. Fisher MB,
    2. Henne KR, and
    3. Boer J
    (2006) The complexities inherent in attempts to decrease drug clearance by blocking sites of CYP-mediated metabolism. Curr Opin Drug Discov Devel 9:101–109.
    OpenUrlPubMed
  7. ↵
    1. Guengerich FP
    (2013) Kinetic deuterium isotope effects in cytochrome P450 oxidation reactions. J Labelled Comp Radiopharm 56:428–431.
    OpenUrlCrossRefPubMed
  8. ↵
    1. Halford B
    (2016) Deuterium swithcheroo breathes life into old drugs. Chem Eng News 94:32–36.
    OpenUrl
  9. ↵
    1. Harbeson SL and
    2. Tung RD
    (2011) Deuterium in drug discovery and development. Annu Rep Med Chem 46:403–417.
    OpenUrl
  10. ↵
    1. Harbeson SL and
    2. Tung RD
    (2014) Deuterium medicinal chemistry: a new approach to drug discovery and development. Med Chem News (Japan) (2)8–22.
  11. ↵
    1. Klein ER and
    2. Klein PD
    1. Horning MG,
    2. Haegele KD,
    3. Sommer KR,
    4. Norwin J, and
    5. Stafford M
    (1976) Metabolic switching of drug pathways as a consequence of deuterium substitution, in Proceedings of the Second International Conference on Stable Isotopes, October 20-23, 1975, Oak Brook, Illinois (Klein ER and Klein PD eds) pp 41-54, US Government Printing Office, Washington, DC.
  12. ↵
    1. Jenks WP
    (1987) Catalysis in Chemistry and Enzymology, Dover Publications Inc., Mineola, NY.
  13. ↵
    Kalydeco. (2015) Package insert. Vertex Pharmaceuticals, Boston, MA.
  14. ↵
    1. Korzekwa KR,
    2. Trager WF, and
    3. Gillette JR
    (1989) Theory for the observed isotope effects from enzymatic systems that form multiple products via branched reaction pathways: cytochrome P-450. Biochemistry 28:9012–9018.
    OpenUrlCrossRefPubMed
  15. ↵
    1. Kushner DJ,
    2. Baker A, and
    3. Dunstall TG
    (1999) Pharmacological uses and perspectives of heavy water and deuterated compounds. Can J Physiol Pharmacol 77:79–88.
    OpenUrlCrossRefPubMed
  16. ↵
    Morgan AJ (2015), inventor, Concert Pharmaceuticals, Inc., assignee. Deuterated CFTR potentiators. U.S. Patent 9,181,192. 10 November 2015.
  17. ↵
    1. Nelson SD and
    2. Trager WF
    (2003) The use of deuterium isotope effects to probe the active site properties, mechanism of cytochrome P450-catalyzed reactions, and mechanisms of metabolically dependent toxicity. Drug Metab Dispos 31:1481–1498.
    OpenUrlAbstract/FREE Full Text
  18. ↵
    1. Northrop DB
    (1975) Steady-state analysis of kinetic isotope effects in enzymic reactions. Biochemistry 14:2644–2651.
    OpenUrlCrossRefPubMed
  19. ↵
    1. Ong T and
    2. Ramsey BW
    (2015) Update in Cystic Fibrosis 2014. Am J Respir Crit Care Med 192:669–675.
    OpenUrl
  20. ↵
    Orkambi. (2016) Package insert. Vertex Pharmaceuticals Incorporated, Boston, MA.
  21. ↵
    Paxil. (2012) Package insert. GlaxoSmithKline, Research Triangle Park, NC.
  22. ↵
    1. Riordan JR
    (2008) CFTR function and prospects for therapy. Annu Rev Biochem 77:701–726.
    OpenUrlCrossRefPubMed
  23. ↵
    1. Rittenberg D and
    2. Schoenheimer R
    (1937) Deuterium as an indicator in the study of intermediate metabolism: XI. Further studies on the biological uptake of deuterium into organic substances, with special reference to fat and cholesterol. J Biol Chem 121:235–253.
    OpenUrlFREE Full Text
  24. ↵
    1. Siracusa CM,
    2. Ryan J,
    3. Burns L,
    4. Wang Y,
    5. Zhang N,
    6. Clancy JP, and
    7. Drotar D
    (2015) Electronic monitoring reveals highly variable adherence patterns in patients prescribed ivacaftor. J Cyst Fibros 14:621–626.
    OpenUrl
  25. ↵
    1. Uttamsingh V,
    2. Gallegos R,
    3. Liu JF,
    4. Harbeson SL,
    5. Bridson GW,
    6. Cheng C,
    7. Wells DS,
    8. Graham PB,
    9. Zelle R, and
    10. Tung R
    (2015) Altering metabolic profiles of drugs by precision deuteration: reducing mechanism-based inhibition of CYP2D6 by paroxetine. J Pharmacol Exp Ther 354:43–54.
    OpenUrlAbstract/FREE Full Text
  26. ↵
    Uttamsingh V, Pilja L, Grotbeck B, Brummel CL, Uddin N, Harbeson SL, Braman V, and Cassella J (2016) CTP-656 tablet confirmed superiority of pharmacokinetic profile relative to Kalydeco in phase 1 clinical studies. J Cyst Fibros 15:S22 (Abstract WS13.6).
  27. ↵
    1. Van Goor F,
    2. Hadida S,
    3. Grootenhuis PD,
    4. Burton B,
    5. Cao D,
    6. Neuberger T,
    7. Turnbull A,
    8. Singh A,
    9. Joubran J,
    10. Hazlewood A, et al.
    (2009) Rescue of CF airway epithelial cell function in vitro by a CFTR potentiator, VX-770. Proc Natl Acad Sci USA 106:18825–18830.
    OpenUrlAbstract/FREE Full Text
  28. ↵
    1. Van Goor F,
    2. Hadida S,
    3. Grootenhuis PD,
    4. Burton B,
    5. Stack JH,
    6. Straley KS,
    7. Decker CJ,
    8. Miller M,
    9. McCartney J,
    10. Olson ER, et al.
    (2011) Correction of the F508del-CFTR protein processing defect in vitro by the investigational drug VX-809. Proc Natl Acad Sci USA 108:18843–18848.
    OpenUrlAbstract/FREE Full Text
  29. ↵
    1. Wainwright CE,
    2. Elborn JS,
    3. Ramsey BW,
    4. Marigowda G,
    5. Huang X,
    6. Cipolli M,
    7. Colombo C,
    8. Davies JC,
    9. De Boeck K,
    10. Flume PA, et al., TRAFFIC Study Group, and TRANSPORT Study Group
    (2015) Lumacaftor-ivacaftor in patients with cystic fibrosis homozygous for Phe508del CFTR. N Engl J Med 373:220–231.
    OpenUrlCrossRefPubMed
  30. ↵
    Yang X, Hadida Ruah S, Grootenjuis P, Van Goor FF, Botfield M, and Zlokarnik G (2010), inventors, Vertex Pharmaceuticals, assignee. Modulators of cystic fibrosis transmembrane conductance regulator. International Patent Publication Number WO 2010/108155. 23 September 2010.
  31. ↵
    Zha J, Zhang J and Ordonez C (2011) Pharmacokinetics (PK), metabolism and excretion of [14C] VX-770, a potentiator of cystic fibrosis transmembrane conductance regulator (CFTR), in healthy subjects. J Clin Pharmacol 51:1358–1359 (Abstract 1124131).
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 362 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 362, Issue 2
1 Aug 2017
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Altering Metabolic Profiles of Drugs by Precision Deuteration 2: Discovery of a Deuterated Analog of Ivacaftor with Differentiated Pharmacokinetics for Clinical Development
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleDrug Discovery and Translational Medicine

CTP-656: Deuterated Ivacaftor Analog with Differentiated PK

Scott L. Harbeson, Adam J. Morgan, Julie F. Liu, Ara M. Aslanian, Sophia Nguyen, Gary W. Bridson, Christopher L. Brummel, Lijun Wu, Roger D. Tung, Lana Pilja, Virginia Braman and Vinita Uttamsingh
Journal of Pharmacology and Experimental Therapeutics August 1, 2017, 362 (2) 359-367; DOI: https://doi.org/10.1124/jpet.117.241497

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleDrug Discovery and Translational Medicine

CTP-656: Deuterated Ivacaftor Analog with Differentiated PK

Scott L. Harbeson, Adam J. Morgan, Julie F. Liu, Ara M. Aslanian, Sophia Nguyen, Gary W. Bridson, Christopher L. Brummel, Lijun Wu, Roger D. Tung, Lana Pilja, Virginia Braman and Vinita Uttamsingh
Journal of Pharmacology and Experimental Therapeutics August 1, 2017, 362 (2) 359-367; DOI: https://doi.org/10.1124/jpet.117.241497
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF + SI
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Glucose transport modulators and mouse behavioral testing
  • Translational PK/PD Modeling of M3258
  • Kidney protective effects of BI 685509
Show more Drug Discovery and Translational Medicine

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics