Article Figures & Data
Figures
- Fig. 1.
Hydrogen peroxide measurements in primary bone cells after stimulation with 25 ng/ml PMA (A) and 50 mM EtOH (B). Statistical differences were determined by Student’s t test; values with different letter subscripts are statistically different from each other (P < 0.05). (C) Design of the feeding studies.
- Fig. 2.
(A) Measurement of serum osteocalcin concentration from 6- and 14-week-old WT and TgCAT mice. Statistical significance was determined by one-way analysis of variance followed by Student-Newman-Keuls post hoc analysis; values with different letter subscripts are statistically different from each other (P < 0.05). (B) Total number of mature osteoclasts from WT and TgCAT bone marrow cells as identified by TRAP staining after culture in media containing 15 ng of RANKL and 20 nM 1,25(OH)2D3 for 10 days. Statistical differences were determined by Student’s t test; values with different letter subscripts are statistically different from each other (P < 0.05). OC, osteocalcin.
- Fig. 3.
MicroCT analysis of tibial trabecular bone—BV/TV percentage (A), Tb.N (B), Tb.Sp (C), and Tb.Th (D)—in 6- and 14-week-old WT and TgCAT mice. Data are expressed with the center line indicating the mean and error bars as ± S.E.M. Statistical significance was determined by two-way analysis of variance followed by Student-Newman-Keuls post hoc analysis. Values with different letter subscripts are statistically different from each other (P < 0.05).
- Fig. 4.
Representative three-dimensional reconstructed images from MicroCT scans of both 6- and 14-week-old WT and TgCAT mice.
- Fig. 5.
MicroCt analysis of tibial cortical bone—cross-sectional area (A), total cross-sectional area (B), periosteal perimeter (C), diameter (D), thickness (E), and endosteal perimeter (F)—in 6- and 14-week-old WT and TgCAT mice. Data are expressed with the center line indicating the mean and error bars as ± S.E.M. Statistical significance was determined by two-way analysis of variance followed by Student-Newman-Keuls post hoc analysis. Values with different letter subscripts are statistically different from each other (P < 0.05).
- Fig. 6.
Assessment of mechanical strength of the femur, peak load (A) and stiffness (B), for chow-fed, PF, and EtOH-fed WT mice and chow-fed, PF, and EtOH-fed TgCAT mice (n = 5/group). Data are expressed as the mean ± S.E.M. Statistical significance was determined by one-way analysis of variance followed by Student-Newman-Keuls post hoc analysis; values with different letter subscripts are statistically different from each other (P < 0.05).
- Fig. 7.
Total mRNA was extracted from 6- and 14-week-old femurs of TgCAT mice, and expression of sclerostin (A), collagen 1 (B), and PPARγ (C) was compared. Statistical differences were determined by Student’s t test; values with different letter subscripts are statistically different from each other (P < 0.05). SOST, Sclerostin.
- Fig. 8.
MicroCT analysis of tibial trabecular bone—BV/TV percentage (A), Tb.N (B), Tb.Sp (C), and Tb.Th (D)—in WT PF mice, WT EtOH-fed mice, TgCAT PF, and TgCAT EtOH-fed mice. Data are expressed with the center line indicating the mean and error bars as ± S.E.M. Statistical significance was determined by two-way analysis of variance followed by Student-Newman-Keuls post hoc analysis. Values with different letter subscripts are statistically different from each other (P < 0.05).
Tables
- TABLE 1
Effects of catalase genotype and EtOH on cortical bone parameters in female mice
pQCT analysis of cortical bone from chow-fed, PF, and EtOH-fed WT mice and chow-fed, PF, and EtOH-fed TgCAT mice (n = 5/group). Data are expressed as the mean with S.E.M. in parentheses. Statistical significance was determined by two-way analysis of variance followed by Student-Newman-Keuls post hoc analysis.
WT TgCAT Chow PF EtOH Chow PF EtOH BMD (mg/cm3) 873.31 (5.73)a 863.31 (5.84)a 815.58 (6.99)b 874.22 (5.73)a 876.84 (5.84)a 858.05 (6.99)c BMC (mg) 0.78 (0.02)a 0.77 (0.02)a 0.65 (0.02)b 0.76 (0.02)a 0.79 (0.02)a 0.72 (0.02)c Total area (mm2) 0.903 (0.02)a 0.898 (0.02)a 0.802 (0.02)b 0.871 (0.02)a 0.904 (0.02)a 0.848 (0.02)b Thickness (mm) 0.279 (0.00)a 0.276 (0.02)a 0.253 (0.00)b 0.284 (0.02)a 0.285 (0.02)a 0.269 (0.02)c BMC, bone mineral content.
Values with different letter subscripts are statistically different from each other (P < 0.05). a>b; a>c; b<c.
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