Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleCardiovascular

Ophiopogonin D Attenuates Doxorubicin-Induced Autophagic Cell Death by Relieving Mitochondrial Damage In Vitro and In Vivo

Ying-Yu Zhang, Chen Meng, Xin-Mu Zhang, Cai-Hua Yuan, Ming-Da Wen, Zhong Chen, Da-Chuan Dong, Yan-Hong Gao, Chang Liu and Zhao Zhang
Journal of Pharmacology and Experimental Therapeutics January 2015, 352 (1) 166-174; DOI: https://doi.org/10.1124/jpet.114.219261
Ying-Yu Zhang
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chen Meng
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xin-Mu Zhang
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cai-Hua Yuan
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ming-Da Wen
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhong Chen
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Da-Chuan Dong
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yan-Hong Gao
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chang Liu
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhao Zhang
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China (Y.-Y.Z., C.M., C.-H.Y., M.-D.W., Z.C., D.-C.D., Y.-H.G., C.L., Z.Z.); Department of Clinical Medicine, Changchun Medical College, Changchun, China (Y.-Y.Z.); and Department of Biopharmaceutical, School of Pharmacy, Jilin University, Changchun, China (X.-M.Z)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

It has been reported that ophiopogonin D (OP-D), a steroidal glycoside and an active component extracted from Ophiopogon japonicas, promotes antioxidative protection of the cardiovascular system. However, it is unknown whether OP-D exerts protective effects against doxorubicin (DOX)-induced autophagic cardiomyocyte injury. Here, we demonstrate that DOX induced excessive autophagy through the generation of reactive oxygen species (ROS) in H9c2 cells and in mouse hearts, which was indicated by a significant increase in the number of autophagic vacuoles, LC3-II/LC3-I ratio, and upregulation of the expression of GFP-LC3. Pretreatment with OP-D partially attenuated the above phenomena, similar to the effects of treatment with 3-methyladenine. In addition, OP-D treatment significantly relieved the disruption of the mitochondrial membrane potential by antioxidative effects through downregulating the expression of both phosphorylated c-Jun N-terminal kinase and extracellular signal-regulated kinase. The ability of OP-D to reduce the generation of ROS due to mitochondrial damage and, consequently, to inhibit autophagic activity partially accounts for its protective effects in the hearts against DOX-induced toxicity.

Footnotes

    • Received August 11, 2014.
    • Accepted November 4, 2014.
  • Y.-Y.Z. and C.M. contributed equally to this work.

  • This work was supported by the National Natural Science Foundation of China [Grants 30871228, 31171302]; the Opening Project of Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology at Nanjing Normal University [Grant 2011MMBKF04]; and the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions [Grant 164320H106]. Z.Z. and C.L. are the associate fellows at the Collaborative Innovation Center for Cardiovascular Disease Translational Medicine of Nanjing Medical University.

  • dx.doi.org/10.1124/jpet.114.219261.

  • Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 352 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 352, Issue 1
1 Jan 2015
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Ophiopogonin D Attenuates Doxorubicin-Induced Autophagic Cell Death by Relieving Mitochondrial Damage In Vitro and In Vivo
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleCardiovascular

Protective Effects of OP-D against DOX-Induced Cardiotoxicity

Ying-Yu Zhang, Chen Meng, Xin-Mu Zhang, Cai-Hua Yuan, Ming-Da Wen, Zhong Chen, Da-Chuan Dong, Yan-Hong Gao, Chang Liu and Zhao Zhang
Journal of Pharmacology and Experimental Therapeutics January 1, 2015, 352 (1) 166-174; DOI: https://doi.org/10.1124/jpet.114.219261

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Research ArticleCardiovascular

Protective Effects of OP-D against DOX-Induced Cardiotoxicity

Ying-Yu Zhang, Chen Meng, Xin-Mu Zhang, Cai-Hua Yuan, Ming-Da Wen, Zhong Chen, Da-Chuan Dong, Yan-Hong Gao, Chang Liu and Zhao Zhang
Journal of Pharmacology and Experimental Therapeutics January 1, 2015, 352 (1) 166-174; DOI: https://doi.org/10.1124/jpet.114.219261
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Anti-apoptotic effect of rosuvastatin via autophagy
  • Improved Assessment of Cardiovascular Safety Data
  • G6PD, DNA methylation, and PAH
Show more Cardiovascular

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2021 by the American Society for Pharmacology and Experimental Therapeutics