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Research ArticleInflammation, Immunopharmacology, and Asthma

Inhibitory Effect of Baicalin on Collagen-Induced Arthritis in Rats through the Nuclear Factor–κB Pathway

Hong-Zhi Wang, Hai-He Wang, Shi-Shun Huang, Hong Zhao, Yong-Gang Cao, Guang-Zhi Wang, Dong Wang, Zhi-Gang Wang and Yan-Hong Liu
Journal of Pharmacology and Experimental Therapeutics August 2014, 350 (2) 435-443; DOI: https://doi.org/10.1124/jpet.114.215145
Hong-Zhi Wang
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Hai-He Wang
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Shi-Shun Huang
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Hong Zhao
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Yong-Gang Cao
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Guang-Zhi Wang
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Dong Wang
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Zhi-Gang Wang
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Yan-Hong Liu
Department of Laboratory Diagnosis (H.-Z.W., Y.-H.L.), The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Laboratory Diagnosis (H.-Z.W., G.-Z.W.,), Department of Respiratory Medicine (H.Z.), and Department of Orthopaedic Surgery (D.W.), The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China; College of Medical Laboratory Science and Technology and the Key Laboratory of Molecular Diagnosis in Laboratory Medicine (H.-H.W., S.-S.H., Z.-G.W.), and Department of Pharmacology (Y.-G.C.), Harbin Medical University, Daqing, China
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Abstract

This study focused on the potential therapeutic effect of baicalin on collagen-induced arthritis (CIA) in rats and the underlying mechanisms. The CIA rats were injected with baicalin (50, 100, or 200 mg/kg) once daily for 30 days. The rats were monitored for clinical severity of arthritis, and joint tissues were used for radiographic assessment and histologic examination. We quantified tumor necrosis factor–α (TNF-α) and interleukin-1β (IL-1β) in experimental animals and used Western blots to assess levels of protein abundance, phosphorylation, and acetylation of nuclear factor (NF)-κB p65 and sirtuin 1 (sirt1) protein expression in joint tissues. Human fibroblast-like synoviocytes from rheumatoid arthritis (HFLS-RA) were adopted in further mechanistic investigations. Baicalin intraperitoneal injection for 30 days dose-dependently blocked clinical manifestations of CIA, such as functional impairment and swollen red paws. Meanwhile, it alleviated collagen-induced joint inflammation injury and inhibited the secretion of TNF-α and IL-1β in both rat synovium and HFLS-RA. Further mechanistic investigations revealed that baicalin suppresses NF-κB p65 protein expression and phosphorylation in synovial tissue and human-derived synoviocytes. Moreover, the acetylation of NF-κB p65 was downregulated by baicalin, which negatively correlates with the baicalin-induced upregulation of sirt1 expression in the same conditions. The data indicate that CIA in rats can be alleviated by baicalin treatment via relieving joint inflammation, which is related to the suppression of synovial NF-κB p65 protein expression and the elevation of its deacetylation by sirt1.

Footnotes

    • Received April 17, 2014.
    • Accepted May 29, 2014.
  • This work was supported by the Heilongjiang Education Department, China [Grant 12541523]; and China Postdoctoral Scientific Foundation [Grants 20100471022 and 201104420].

  • dx.doi.org/10.1124/jpet.114.215145.

  • Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 350 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 350, Issue 2
1 Aug 2014
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Research ArticleInflammation, Immunopharmacology, and Asthma

Therapeutic Effects of Baicalin on CIA in Rats

Hong-Zhi Wang, Hai-He Wang, Shi-Shun Huang, Hong Zhao, Yong-Gang Cao, Guang-Zhi Wang, Dong Wang, Zhi-Gang Wang and Yan-Hong Liu
Journal of Pharmacology and Experimental Therapeutics August 1, 2014, 350 (2) 435-443; DOI: https://doi.org/10.1124/jpet.114.215145

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Research ArticleInflammation, Immunopharmacology, and Asthma

Therapeutic Effects of Baicalin on CIA in Rats

Hong-Zhi Wang, Hai-He Wang, Shi-Shun Huang, Hong Zhao, Yong-Gang Cao, Guang-Zhi Wang, Dong Wang, Zhi-Gang Wang and Yan-Hong Liu
Journal of Pharmacology and Experimental Therapeutics August 1, 2014, 350 (2) 435-443; DOI: https://doi.org/10.1124/jpet.114.215145
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