Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleGastrointestinal, Hepatic, Pulmonary, and Renal

Protective Effects of Acetaminophen on Ibuprofen-Induced Gastric Mucosal Damage in Rats with Associated Suppression of Matrix Metalloproteinase

Eriko Fukushima, Noriyuki Monoi, Shigeo Mikoshiba, Yutaka Hirayama, Tetsushi Serizawa, Kiyo Adachi, Misao Koide, Motoyasu Ohdera, Michiaki Murakoshi and Hisanori Kato
Journal of Pharmacology and Experimental Therapeutics April 2014, 349 (1) 165-173; DOI: https://doi.org/10.1124/jpet.113.210021
Eriko Fukushima
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Noriyuki Monoi
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shigeo Mikoshiba
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yutaka Hirayama
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsushi Serizawa
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiyo Adachi
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Misao Koide
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Motoyasu Ohdera
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michiaki Murakoshi
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hisanori Kato
Research and Development Headquarters, Lion Corporation, Kanagawa, Japan (E.F., N.M., S.M., Y.H., T.S., K.A., M.K., M.O., M.M.); and Organization for Interdisciplinary Research Projects, University of Tokyo, Tokyo, Japan (H.K.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to cause gastric mucosal damage as a side effect. Acetaminophen, widely used as an analgesic and antipyretic drug, has gastroprotective effects against gastric lesions induced by absolute ethanol and certain NSAIDs. However, the mechanisms that underlie the gastroprotective effects of acetaminophen have not yet been clarified. In the present study, we examined the role and protective mechanism of acetaminophen on ibuprofen-induced gastric damage in rats. Ibuprofen and acetaminophen were administered orally, and the gastric mucosa was macroscopically examined 4 hours later. Acetaminophen decreased ibuprofen-induced gastric damage in a dose-dependent manner. To investigate the mechanisms involved, transcriptome analyses of the ibuprofen-damaged gastric mucosa were performed in the presence and absence of acetaminophen. Ingenuity pathway analysis (IPA) software revealed that acetaminophen suppressed the pathways related to cellular assembly and inflammation, whereas they were highly activated by ibuprofen. On the basis of gene classifications from the IPA Knowledge Base, we identified the following five genes that were related to gastric damage and showed significant changes in gene expression: interleukin-1β (IL-1β), chemokine (C–C motif) ligand 2 (CCL2), matrix metalloproteinase-10 (MMP-10), MMP-13, and FBJ osteosarcoma oncogene (FOS). Expression of these salient genes was confirmed using real-time polymerase chain reaction. The expression of MMP-13 was the most reactive to the treatments, showing strong induction by ibuprofen and suppression by acetaminophen. Moreover, MMP-13 inhibitors decreased ibuprofen-induced gastric damage. In conclusion, these results suggest that acetaminophen decreases ibuprofen-induced gastric mucosal damage and that the suppression of MMP-13 may play an important role in the gastroprotective effects of acetaminophen.

Footnotes

    • Received September 26, 2013.
    • Accepted February 3, 2014.
  • dx.doi.org/10.1124/jpet.113.210021.

  • Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 349 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 349, Issue 1
1 Apr 2014
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Protective Effects of Acetaminophen on Ibuprofen-Induced Gastric Mucosal Damage in Rats with Associated Suppression of Matrix Metalloproteinase
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleGastrointestinal, Hepatic, Pulmonary, and Renal

Acetaminophen Reduces Ibuprofen-Induced Gastric Damage

Eriko Fukushima, Noriyuki Monoi, Shigeo Mikoshiba, Yutaka Hirayama, Tetsushi Serizawa, Kiyo Adachi, Misao Koide, Motoyasu Ohdera, Michiaki Murakoshi and Hisanori Kato
Journal of Pharmacology and Experimental Therapeutics April 1, 2014, 349 (1) 165-173; DOI: https://doi.org/10.1124/jpet.113.210021

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleGastrointestinal, Hepatic, Pulmonary, and Renal

Acetaminophen Reduces Ibuprofen-Induced Gastric Damage

Eriko Fukushima, Noriyuki Monoi, Shigeo Mikoshiba, Yutaka Hirayama, Tetsushi Serizawa, Kiyo Adachi, Misao Koide, Motoyasu Ohdera, Michiaki Murakoshi and Hisanori Kato
Journal of Pharmacology and Experimental Therapeutics April 1, 2014, 349 (1) 165-173; DOI: https://doi.org/10.1124/jpet.113.210021
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • MIP3α in Progressive Renal Injury Associated with Obesity
  • A Novel Long-Acting GLP-2, HM15912, for Short Bowel Syndrome
  • H2S Overproduction and Colonic Hypomotility in DM
Show more Gastrointestinal, Hepatic, Pulmonary, and Renal

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics