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Research ArticleBehavioral Pharmacology

Quantitative Analyses of Antagonism: Combinations of Midazolam and Either Flunitrazepam or Pregnanolone in Rhesus Monkeys Discriminating Midazolam

Lisa R. Gerak and Charles P. France
Journal of Pharmacology and Experimental Therapeutics March 2012, 340 (3) 742-749; DOI: https://doi.org/10.1124/jpet.111.188250
Lisa R. Gerak
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Charles P. France
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Abstract

Adverse effects of benzodiazepines limit their clinical use; these effects might be reduced without altering therapeutic effects by administering other positive GABAA modulators (i.e., neuroactive steroids) with benzodiazepines. One concern with this strategy involves reversing these combined effects in case of overdose. The current study examined whether flumazenil can attenuate the combined effects of two benzodiazepines, midazolam and flunitrazepam, and the combined effects of midazolam and the neuroactive steroid pregnanolone, in four monkeys discriminating midazolam. Each positive modulator produced ≥80% midazolam-lever responding. Interactions between midazolam and either flunitrazepam or pregnanolone were additive. Flumazenil antagonized the benzodiazepines when they were administered alone or in combination. Schild analyses yielded slopes that did not deviate from unity, regardless of whether benzodiazepines were administered alone or together; the pA2 value for flumazenil was 7.58. In contrast, flumazenil enhanced the effects of pregnanolone with 0.32 mg/kg flumazenil shifting the pregnanolone dose-effect curve 2-fold leftward. Flumazenil attenuated the combined effects of midazolam and pregnanolone, although antagonism was not dose-dependent. Thus, the interaction between two benzodiazepines was similar to that of a benzodiazepine and a neuroactive steroid; however, flumazenil more efficiently attenuated a combination of two benzodiazepines compared with a combination of a benzodiazepine and a neuroactive steroid. Although the magnitude of antagonism of a benzodiazepine combined with a neuroactive steroid was reduced, these results support continued exploration of the use of combinations of positive modulators to enhance therapeutic effects while reducing adverse effects.

Footnotes

  • This work was supported by the National Institutes of Health National Institute on Drug Abuse [Grant DA009157 (to L.R.G.), Senior Scientist Award K05-DA17918 (to C.P.F.)].

  • Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.

    http://dx.doi.org/10.1124/jpet.111.188250.

  • ABBREVIATIONS:

    CI
    confidence interval
    n.s.
    not significant.

  • Received September 20, 2011.
  • Accepted December 14, 2011.
  • Copyright © 2012 by The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 340 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 340, Issue 3
1 Mar 2012
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Research ArticleBehavioral Pharmacology

Combinations of Positive GABAA Modulators

Lisa R. Gerak and Charles P. France
Journal of Pharmacology and Experimental Therapeutics March 1, 2012, 340 (3) 742-749; DOI: https://doi.org/10.1124/jpet.111.188250

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Research ArticleBehavioral Pharmacology

Combinations of Positive GABAA Modulators

Lisa R. Gerak and Charles P. France
Journal of Pharmacology and Experimental Therapeutics March 1, 2012, 340 (3) 742-749; DOI: https://doi.org/10.1124/jpet.111.188250
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