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Research ArticleNeuropharmacology

Chronic Ethanol and Withdrawal Differentially Modulate Lateral/Basolateral Amygdala Paracapsular and Local GABAergic Synapses

Marvin R. Diaz, Daniel T. Christian, Nancy J. Anderson and Brian A. McCool
Journal of Pharmacology and Experimental Therapeutics April 2011, 337 (1) 162-170; DOI: https://doi.org/10.1124/jpet.110.177121
Marvin R. Diaz
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Daniel T. Christian
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Nancy J. Anderson
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Brian A. McCool
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Abstract

Withdrawal-related anxiety is cited as a major contributor to relapse in recovering alcoholics. Changes in lateral/basolateral amygdala (BLA) neurotransmission could directly influence anxiety-like behaviors after chronic ethanol exposure and withdrawal. We have shown that these treatments enhance BLA glutamatergic function and neurotransmission. However, the BLA GABAergic system tightly controls the expression of anxiety-like behavior, and additional neuroadaptations in this system are potentially important as well. The intrinsic BLA GABAergic system consists of at least two populations of interneurons: local feed-back interneurons scattered throughout the region and feed-forward interneurons concentrated within groups found in the lateral/paracapsular region of the BLA. In the present study, we found that withdrawal from chronic ethanol robustly decreased presynaptic function at feed-forward GABA synapses but did not alter neurotransmitter release from local interneurons. Differential presynaptic changes at these synapses were complemented by decreased zolpidem sensitivity at feed-forward synapses and decreased midazolam sensitivity at local synapses. Consistent with this, chronic ethanol/withdrawal decreased expression of GABA α1-subunit total protein and increased surface expression of α4-subunit protein. We also found transient increases in GABA-receptor-associated protein levels and persistent increases in γ2-subunit and gephyrin proteins that would suggest alterations in GABAA receptor trafficking that might help regulate changes in α4-subunit localization. These data together suggest that chronic ethanol and withdrawal differentially modulate local and lateral paracapsular cell GABAergic synapses via distinct presynaptic and postsynaptic mechanisms. These findings extend our understanding of the neurobiological mechanisms governing changes in anxiety-like behavior after chronic ethanol exposure and withdrawal.

Footnotes

  • This work was supported by the National Institutes of Health National Institute on Alcohol Abuse and Alcoholism [Grants R01/=/AA014445, U01/=/AA016671, F31/=/AA017576].

  • Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.

    doi:10.1124/jpet.110.177121.

  • ABBREVIATIONS:

    BLA
    lateral/basolateral amygdala
    BS3
    bis(sulfosuccinimidyl)suberate
    CIE
    chronic intermittent ethanol
    GABA-RAP
    GABA receptor-associated protein
    LPC
    lateral paracapsular cell
    IPSC
    inhibitory postsynaptic current
    mIPSC
    miniature IPSC
    WD
    withdrawal
    aCSF
    artificial cerebrospinal fluid
    ANOVA
    analysis of variance
    TTX
    tetrodotoxin
    CON
    control
    QX314
    N-(2,6-dimethylphenylcarbamoylmethyl)triethylammonium bromide
    TBS-T
    Tris-buffered saline
    CGP55845
    2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid hydrochloride
    SCH50911
    2-[(2S)-(+)−5,5-dimethylmorpholin-2-yl]acetic acid.

  • Received November 9, 2010.
  • Accepted January 3, 2011.
  • Copyright © 2011 by The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 387 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 387, Issue 1
1 Oct 2023
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Research ArticleNeuropharmacology

Chronic Ethanol and Withdrawal Differentially Modulate Lateral/Basolateral Amygdala Paracapsular and Local GABAergic Synapses

Marvin R. Diaz, Daniel T. Christian, Nancy J. Anderson and Brian A. McCool
Journal of Pharmacology and Experimental Therapeutics April 1, 2011, 337 (1) 162-170; DOI: https://doi.org/10.1124/jpet.110.177121

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Research ArticleNeuropharmacology

Chronic Ethanol and Withdrawal Differentially Modulate Lateral/Basolateral Amygdala Paracapsular and Local GABAergic Synapses

Marvin R. Diaz, Daniel T. Christian, Nancy J. Anderson and Brian A. McCool
Journal of Pharmacology and Experimental Therapeutics April 1, 2011, 337 (1) 162-170; DOI: https://doi.org/10.1124/jpet.110.177121
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