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Research ArticleTOXICOLOGY

Ascorbylperoxide Contaminating Parenteral Nutrition Perturbs the Lipid Metabolism in Newborn Guinea Pig

Raffi Maghdessian, François Côté, Thérèse Rouleau, Ali Ben Djoudi Ouadda, Émile Levy and Jean-Claude Lavoie
Journal of Pharmacology and Experimental Therapeutics July 2010, 334 (1) 278-284; DOI: https://doi.org/10.1124/jpet.110.166223
Raffi Maghdessian
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François Côté
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Thérèse Rouleau
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Ali Ben Djoudi Ouadda
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Émile Levy
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Jean-Claude Lavoie
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Abstract

The light exposure of parenteral nutritive solutions generates peroxides such as H2O2 and ascorbylperoxide [2,3-diketo-4-hydoxyperoxyl-5,6-dihydroxyhexanoic acid]. This absence of photoprotection is associated with higher plasma triacylglycerol (TG) concentration in premature infants and oxidative stress and H2O2-independent hepatic steatosis in animals. We hypothesized that ascorbylperoxide is the active agent leading to high TG. The aim was to investigate the role of ascorbylperoxide in glucose and lipid metabolism in an animal model of neonatal parenteral nutrition. Three-day-old guinea pigs received through a catheter in the jugular solutions containing dextrose plus 0, 90, 225, or 450 μM ascorbylperoxide. After 4 days, blood and liver were sampled and treated for determinations of TG, cholesterol, markers of oxidative stress (redox potential of glutathione and F2α-isoprostane), and activities and protein levels of acetyl-CoA carboxylase (ACC), glucokinase, and phosphofructokinase (PFK). Ascorbylperoxide concentration was measured in urine on the last day. Data were compared by analysis of variance (p < 0.05). Plasma TG and cholesterol and hepatic PFK activity increased (200% of control), whereas ACC activity decreased (66% of control) in the function of the amount of ascorbylperoxide infused. Both markers of oxidative stress were higher in animals receiving the highest amounts of ascorbylperoxide. The logarithmic relations between urinary ascorbylperoxide and plasma TG (r2 = 0.69) and hepatic PFK activity (r2 = 0.26) were positive, whereas they were negative with ACC activity (r2 = 0.50). In conclusion, ascorbylperoxide contaminating parenteral nutrition stimulates glycolysis, allowing higher availability of substrates for lipid synthesis. The logarithmic relation between urinary ascorbylperoxide and plasma TG suggests a very low efficient concentration.

Footnotes

  • This work was supported by the Canadian Institutes of Health Research [Grant MOP 77637]. R.M. was supported by the Hôpital de la Tour, Meyrin, Switzerland.

  • Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.

    doi:10.1124/jpet.110.166223.

  • ABBREVIATIONS:

    ascorbylperoxide
    2,3-diketo-4-hydoxyperoxyl-5,6-dihydroxyhexanoic acid
    ACC
    acetyl-CoA carboxylase
    GK
    glucokinase
    GSH
    reduced form of glutathione
    GSSG
    disulfide form of glutathione
    PFK
    phosphofrutokinase
    TG
    triacylglyceride
    OTC
    l-2-oxo-thiazolidine 4-carboxilic acid.

  • Received January 20, 2010.
  • Accepted April 5, 2010.
  • Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 385 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 385, Issue 1
1 Apr 2023
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Research ArticleTOXICOLOGY

Ascorbylperoxide Contaminating Parenteral Nutrition Perturbs the Lipid Metabolism in Newborn Guinea Pig

Raffi Maghdessian, François Côté, Thérèse Rouleau, Ali Ben Djoudi Ouadda, Émile Levy and Jean-Claude Lavoie
Journal of Pharmacology and Experimental Therapeutics July 1, 2010, 334 (1) 278-284; DOI: https://doi.org/10.1124/jpet.110.166223

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Research ArticleTOXICOLOGY

Ascorbylperoxide Contaminating Parenteral Nutrition Perturbs the Lipid Metabolism in Newborn Guinea Pig

Raffi Maghdessian, François Côté, Thérèse Rouleau, Ali Ben Djoudi Ouadda, Émile Levy and Jean-Claude Lavoie
Journal of Pharmacology and Experimental Therapeutics July 1, 2010, 334 (1) 278-284; DOI: https://doi.org/10.1124/jpet.110.166223
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