Abstract
The present study was designed to investigate the role of bradykinin (BK) in the knee joint osteoarthritis induced by intra-articular (i.ar.) administration of monosodium iodoacetate (MIA) in the rat, and to determine the efficacy of the kinin B2 receptor antagonists, 4-(S)-amino-5-(4-{4-[2,4-dichloro-3-(2,4-dimethyl-8-quinolyloxymethyl)phenylsulfonamido]-tetrahydro-2H-4-pyranylcarbonyl} piperazino)-5-oxopentyl](trimethyl)ammonium chloride hydrochloride (MEN16132) and icatibant, in reducing pain. Rats received MIA (1 mg/25 μl i.ar.) in the right knee. MEN16132, icatibant (1, 3, and 10 μg/25 μl i.ar.), or saline were administered 7 days after MIA treatment, and their antinociceptive effect was observed for 2 weeks. MEN16132 induced a marked and sustained reduction of incapacitation produced by MIA, approximately 56% inhibition of pain at 3 μg/knee. MEN16132 analgesia was more potent and longer lasting, up to 10 days, than icatibant. MEN16132 (3 μg/knee), at different time points from MIA treatment in separate groups of animals, produced comparable maximal antinociceptive effects, whereas the pain response induced by MIA was unaffected if MEN16132 (10 μg/knee) was administered in the contralateral knee. Indomethacin at high doses (100–625 μg/knee) inhibited by approximately 40% but with a short duration the MIA-induced pain. MIA treatment produced a significant increase of BK and prostaglandin E2 (PGE2) metabolite levels in synovial fluid up to 21 days, and PGE2 metabolite levels were reduced almost to basal values by MEN16132. In conclusion the potent and long-lasting analgesic effect of MEN16132 in MIA-induced osteoarthritis indicates an important role for BK in osteoarthritic pain, and suggests that MEN16132 can be a candidate for the treatment of this chronic disease.
Footnotes
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Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.
doi:10.1124/jpet.109.159657
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ABBREVIATIONS:
- OA
- osteoarthritis
- ANOVA
- analysis of variance
- MIA
- monosodium iodoacetate
- BK
- bradykinin
- i.ar.
- intra-articular
- MEN16132
- 4-(S)-amino-5-(4-{4-[2,4-dichloro-3-(2,4-dimethyl-8-quinolyloxymethyl)phenylsulfonamido]-tetrahydro-2H-4-pyranylcarbonyl} piperazino)-5-oxopentyl](trimethyl)ammonium chloride hydrochloride
- NSAIDs
- nonsteroidal anti-inflammatory drugs
- COX
- cyclooxygenase
- PGE2
- prostaglandin E2
- PGEM
- prostaglandin E2 metabolite
- PBS-T
- phosphate-buffered saline with Tween 20.
- Received July 30, 2009.
- Accepted September 9, 2009.
- © 2009 by The American Society for Pharmacology and Experimental Therapeutics
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