Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Identification and Quantification of 2′,3′-cAMP Release by the Kidney

Jin Ren, Zaichuan Mi, Nicolas A. Stewart and Edwin K. Jackson
Journal of Pharmacology and Experimental Therapeutics March 2009, 328 (3) 855-865; DOI: https://doi.org/10.1124/jpet.108.146712
Jin Ren
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zaichuan Mi
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicolas A. Stewart
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edwin K. Jackson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

This article has a correction. Please see:

  • Correction to “Identification and Quantification of 2′,3′-cAMP Release by the Kidney” - April 01, 2009

Abstract

We recently developed a sensitive assay for 3′,5′-cAMP using high-performance liquid chromatography-tandem mass spectrometry. Using this assay, we investigated the release of 3′,5′-cAMP from isolated, perfused rat kidneys. To our surprise, we observed a dominant chromatographic peak that was because of an endogenous substance that had the same parent ion as 3′,5′-cAMP and that fragmented to the same daughter ion (adenine) as 3′,5′-cAMP. However, the retention time of this unknown was approximately 2.9 min, compared with 6.3 min for authentic 3′,5′-cAMP. We hypothesized that the unknown substance was an isomer of 3′,5′-cAMP. The unknown substance had the same retention time and mass spectral properties as authentic 2′,3′-cAMP. Renal venous secretion of 2′,3′-cAMP was greater in kidneys from 20-week-old genetically hypertensive rats compared with age-matched normotensive rats (12.49 ± 2.14 versus 5.32 ± 1.97 ng/min/g kidney weight, respectively; n = 18). Isoproterenol (1 μM; β-adrenoceptor agonist) increased renal venous 3′,5′-cAMP secretion (approximately 690% of control) but had no effect on 2′,3′-cAMP production. In contrast, rapamycin (0.2 μM; activator of mRNA turnover) and iodoacetate + 2,4-dinitrophenol (50 μM; metabolic inhibitors) increased the renal venous secretion of 2′,3′-cAMP (approximately 1000 and 4100% of control, respectively) while simultaneously decreasing the renal venous secretion of 3′,5′-cAMP. In conclusion, 2′,3′-cAMP is a naturally occurring isomer of 3′,5′-cAMP that is: 1) not made by adenylyl cyclase; 2) released from kidneys into the extracellular compartment; 3) released more by kidneys from rats with long-standing hypertension; 4) derived from mRNA turnover; and 5) increased by energy depletion.

Footnotes

  • This work was supported by the National Institutes of Health [Grants HL69846, DK068575, DK079307].

  • doi:10.1124/jpet.108.146712.

  • ABBREVIATIONS: MRP, multidrug resistance protein; SHR, spontaneously hypertensive rat; WKY, Wistar-Kyoto rat; LC, liquid chromatography; MS/MS, tandem mass spectrometry; SRM, selective reaction monitoring; ARE, AU-rich element.

    • Received September 26, 2008.
    • Accepted November 24, 2008.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 377 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 377, Issue 2
1 May 2021
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Identification and Quantification of 2′,3′-cAMP Release by the Kidney
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Identification and Quantification of 2′,3′-cAMP Release by the Kidney

Jin Ren, Zaichuan Mi, Nicolas A. Stewart and Edwin K. Jackson
Journal of Pharmacology and Experimental Therapeutics March 1, 2009, 328 (3) 855-865; DOI: https://doi.org/10.1124/jpet.108.146712

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Identification and Quantification of 2′,3′-cAMP Release by the Kidney

Jin Ren, Zaichuan Mi, Nicolas A. Stewart and Edwin K. Jackson
Journal of Pharmacology and Experimental Therapeutics March 1, 2009, 328 (3) 855-865; DOI: https://doi.org/10.1124/jpet.108.146712
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Increased momentary urethral closure by 5-HT2C agonist
  • Canalicular Transporters Studied with Fluorescent Bile Acids
  • Knockout of Add3 Promotes L-NAME–Induced Renal Injury
Show more Gastrointestinal, Hepatic, Pulmonary, and Renal

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2021 by the American Society for Pharmacology and Experimental Therapeutics