Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleINFLAMMATION AND IMMUNOPHARMACOLOGY

S-Adenosyl-l-homocysteine Hydrolase Inactivation Curtails Ovalbumin-Induced Immune Responses

Yun-Feng Fu, Jun-Xia Wang, Yang Zhao, Yang Yang, Wei Tang, Jia Ni, Yi-Na Zhu, Ru Zhou, Pei-Lan He, Chuan Li, Xiao-Yu Li, Yi-Fu Yang, Brian R. Lawson and Jian-Ping Zuo
Journal of Pharmacology and Experimental Therapeutics March 2006, 316 (3) 1229-1237; DOI: https://doi.org/10.1124/jpet.105.093369
Yun-Feng Fu
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun-Xia Wang
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yang Zhao
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yang Yang
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei Tang
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jia Ni
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yi-Na Zhu
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ru Zhou
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pei-Lan He
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chuan Li
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiao-Yu Li
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yi-Fu Yang
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brian R. Lawson
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jian-Ping Zuo
Laboratory of Immunopharmacology and State Key Laboratory of Drug Research (Y.-F.F., J.-X.W., W.T., J.N., Y.-N.Z., R.Z., P.-L.H., X.-Y.L., Y.-F.Y., J.-P.Z.), Center for Drug Metabolism and Pharmacokinetics Research (Y.Z., C.L.), Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People's Republic of China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China (Y.Y., J.-P.Z.); and Diazyme Laboratories Division General Atomics, San Diego, California (B.R.L.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The reversible S-adenosyl-l-homocysteine (AdoHcy) hydrolase inhibitor methyl 4-(adenin-9-yl)-2-hydroxybutanoate (DZ2002) suppresses macrophage activation and function. The effects of DZ2002 on T cell function, however, are still unclear. Here, we examined whether DZ2002 alters type 1 helper T cell (Th1) and/or type 2 helper T cell (Th2) immune responses, and whether these effects are associated with both the inhibition of AdoHcy hydrolase and intracellular elevation of endogenous AdoHcy. Male C57BL/6 mice immunized with ovalbumin (OVA) were treated with DZ2002 (1, 5, and 25 mg/kg/day) after which lymphocyte proliferation, cytokine production, and IgG responses to OVA were monitored. Administration of DZ2002 dose dependently suppressed OVA-specific lymphocyte proliferation and anti-OVA IgG production compared with controls. Interleukin (IL)-2 and interferon (IFN)-γ as well as anti-OVA IgG2a and IgG3, indicators of Th1 immune responses, were markedly decreased in mice treated with DZ2002, whereas IL-4 and anti-OVA IgG1, indicators of Th2 immune responses, were only mildly suppressed. AdoHcy hydrolase activity in spleens of DZ2002-treated mice was substantially blocked, and not surprisingly, AdoHcy levels were significantly elevated compared with controls. Finally, similar immunosuppressive effects were also observed in mice treated with AdoHcy. These data strongly indicate that DZ2002 suppresses antigen-induced specific immune responses, particularly Th1 responses, through inhibition of AdoHcy hydrolase and elevation of endogenous AdoHcy.

  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 316 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 316, Issue 3
1 Mar 2006
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
S-Adenosyl-l-homocysteine Hydrolase Inactivation Curtails Ovalbumin-Induced Immune Responses
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleINFLAMMATION AND IMMUNOPHARMACOLOGY

S-Adenosyl-l-homocysteine Hydrolase Inactivation Curtails Ovalbumin-Induced Immune Responses

Yun-Feng Fu, Jun-Xia Wang, Yang Zhao, Yang Yang, Wei Tang, Jia Ni, Yi-Na Zhu, Ru Zhou, Pei-Lan He, Chuan Li, Xiao-Yu Li, Yi-Fu Yang, Brian R. Lawson and Jian-Ping Zuo
Journal of Pharmacology and Experimental Therapeutics March 1, 2006, 316 (3) 1229-1237; DOI: https://doi.org/10.1124/jpet.105.093369

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleINFLAMMATION AND IMMUNOPHARMACOLOGY

S-Adenosyl-l-homocysteine Hydrolase Inactivation Curtails Ovalbumin-Induced Immune Responses

Yun-Feng Fu, Jun-Xia Wang, Yang Zhao, Yang Yang, Wei Tang, Jia Ni, Yi-Na Zhu, Ru Zhou, Pei-Lan He, Chuan Li, Xiao-Yu Li, Yi-Fu Yang, Brian R. Lawson and Jian-Ping Zuo
Journal of Pharmacology and Experimental Therapeutics March 1, 2006, 316 (3) 1229-1237; DOI: https://doi.org/10.1124/jpet.105.093369
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Lipopolysaccharide Induces Epithelium- and Prostaglandin E2-Dependent Relaxation of Mouse Isolated Trachea through Activation of Cyclooxygenase (COX)-1 and COX-2
  • Cannabinoid-Mediated Elevation of Intracellular Calcium: A Structure-Activity Relationship
  • Protease-Activated Receptor-2 Peptides Activate Neurokinin-1 Receptors in the Mouse Isolated Trachea
Show more INFLAMMATION AND IMMUNOPHARMACOLOGY

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics