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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Chemoresistance to Depsipeptide FK228 [(E)-(1S,4S,10S,21R)-7-[(Z)-Ethylidene]-4,21-diisopropyl-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8,7,6]-tricos-16-ene-3,6,9,22-pentanone] Is Mediated by Reversible MDR1 Induction in Human Cancer Cell Lines

Jim J. Xiao, Ying Huang, Zunyan Dai, Wolfgang Sadée, Jiyun Chen, Shujun Liu, Guido Marcucci, John Byrd, Joseph M. Covey, John Wright, Michael Grever and Kenneth K. Chan
Journal of Pharmacology and Experimental Therapeutics July 2005, 314 (1) 467-475; DOI: https://doi.org/10.1124/jpet.105.083956
Jim J. Xiao
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Ying Huang
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Zunyan Dai
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Wolfgang Sadée
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Jiyun Chen
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Shujun Liu
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Guido Marcucci
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John Byrd
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Joseph M. Covey
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John Wright
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Michael Grever
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Kenneth K. Chan
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Abstract

Histone acetylation status, an epigenetic determinant of gene transcription, is controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). The potent HDAC inhibitor FK228 [(E)-(1S,4S,10S,21R)-7-[(Z)-ethylidene]-4,21-diisopropyl-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8,7,6]-tricos-16-ene-3,6,9,22-pentanone] is a substrate for multidrug resistance protein (MDR1) and multidrug resistance-associated protein 1 (MRP1), both of which mediate FK228 resistance. To determine the mechanisms underlying acquired FK228 resistance, we developed four FK228-resistant cell lines from HCT-15, IGROV1, MCF7, and K562 cells by stepwise increases in FK228 exposure. Parent and resistant cells were characterized using a 70-oligomer cDNA microarray, real-time reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and cytotoxicity assays. At both mRNA and protein levels, MDR1, but not MRP1 or other potential resistance genes, was strongly up-regulated in all resistant cell lines. HAT or HDAC activities were unaffected in resistant cells, consistent with a lack of cross-resistance to HDAC inhibitors that are not MDR1 substrates. FK228 was found to reversibly induce MDR1 expression by HDAC inhibition and subsequent histone hyperacetylation at the MDR1 promoter, as shown by real-time RT-PCR, Western blot, and chromatin immunoprecipitation. This study reveals a significant role of histone acetylation in MDR1 transcription, which seems to mediate FK228 resistance.

Footnotes

  • This work was supported by National Institutes of Health Grant 1R21CA 96323 and by BioMedical Mass Spectrometry Laboratory at The Ohio State University

  • Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.

  • doi:10.1124/jpet.105.083956.

  • ABBREVIATIONS: HAT, histone acetyltransferase; HDAC, histone deacetylase; FK228, depsipeptide or FR901228, (E)-(1S,4S,10S,21R)-7-[(Z)-ethylidene]-4,21-diisopropyl-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8,7,6]-tricos-16-ene-3,6,9,22-pentanone; ABC, ATP-binding cassette; RT-PCR, reverse transcription-polymerase chain reaction; ChIP, chromatin immunoprecipitation; TSA, trichostatin A; SAHA, suberoylanilide hydroxamic acid; CsA, cyclosporin A.

    • Received January 19, 2005.
    • Accepted April 13, 2005.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 384 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 384, Issue 2
1 Feb 2023
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Chemoresistance to Depsipeptide FK228 [(E)-(1S,4S,10S,21R)-7-[(Z)-Ethylidene]-4,21-diisopropyl-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8,7,6]-tricos-16-ene-3,6,9,22-pentanone] Is Mediated by Reversible MDR1 Induction in Human Cancer Cell Lines
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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Chemoresistance to Depsipeptide FK228 [(E)-(1S,4S,10S,21R)-7-[(Z)-Ethylidene]-4,21-diisopropyl-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8,7,6]-tricos-16-ene-3,6,9,22-pentanone] Is Mediated by Reversible MDR1 Induction in Human Cancer Cell Lines

Jim J. Xiao, Ying Huang, Zunyan Dai, Wolfgang Sadée, Jiyun Chen, Shujun Liu, Guido Marcucci, John Byrd, Joseph M. Covey, John Wright, Michael Grever and Kenneth K. Chan
Journal of Pharmacology and Experimental Therapeutics July 1, 2005, 314 (1) 467-475; DOI: https://doi.org/10.1124/jpet.105.083956

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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Chemoresistance to Depsipeptide FK228 [(E)-(1S,4S,10S,21R)-7-[(Z)-Ethylidene]-4,21-diisopropyl-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8,7,6]-tricos-16-ene-3,6,9,22-pentanone] Is Mediated by Reversible MDR1 Induction in Human Cancer Cell Lines

Jim J. Xiao, Ying Huang, Zunyan Dai, Wolfgang Sadée, Jiyun Chen, Shujun Liu, Guido Marcucci, John Byrd, Joseph M. Covey, John Wright, Michael Grever and Kenneth K. Chan
Journal of Pharmacology and Experimental Therapeutics July 1, 2005, 314 (1) 467-475; DOI: https://doi.org/10.1124/jpet.105.083956
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