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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Estrogen Regulation of the Cytochrome P450 3A Subfamily in Humans

Eric T. Williams, Malgorzata Leyk, Steven A. Wrighton, Peter J. A. Davies, David S. Loose, Gregory L. Shipley and Henry W. Strobel
Journal of Pharmacology and Experimental Therapeutics November 2004, 311 (2) 728-735; DOI: https://doi.org/10.1124/jpet.104.068908
Eric T. Williams
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Malgorzata Leyk
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Steven A. Wrighton
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Peter J. A. Davies
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David S. Loose
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Gregory L. Shipley
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Henry W. Strobel
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Abstract

This study examines the possible role of estrogen in regulating the expression of the human CYP3A subfamily: CYP3A4, CYP3A5, CYP3A7, and CYP3A43. To accomplish this goal, mRNA was quantified from human livers and endometrial samples, and total CYP3A protein levels were evaluated by Western immunoblot analysis of the liver samples. The human endometrial samples were from premenopausal and postmenopausal women. The premenopausal endometrium was either in the proliferative or secretory phase, whereas for the postmenopausal endometrium samples, the women had been treated with either a placebo or estropipate, an estrogen substitute. After analyses, CYP3A4 mRNA was shown to have lower hepatic expression in females than in males. In the endometrium, CYP3A4 and CYP3A43 are down-regulated by estrogen, whereas CYP3A5 is expressed at higher levels during the secretory phase. CYP3A7 was not detected in the endometrium. In addition, the CYP3A subfamily showed increased mRNA expression in the liver as age increased. The expression levels of total CYP3A protein and total CYP3A mRNA showed good correlation. Despite apparent regulation of CYP3A4 mRNA expression by estrogen, the effects of estrogen may be overshadowed by additional regulators of gene expression.

Footnotes

  • This study was supported by the Women's Fund for Health Education and Research, 2002; Houston, Texas.

  • doi:10.1124/jpet.104.068908.

  • ABBREVIATIONS: P450, cytochrome P450; PCR, polymerase chain reaction; NTC, no template control; NAC, no amplification control; PXR, pregnane X receptor.

    • Received April 27, 2004.
    • Accepted July 21, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 311 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 311, Issue 2
1 Nov 2004
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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Estrogen Regulation of the Cytochrome P450 3A Subfamily in Humans

Eric T. Williams, Malgorzata Leyk, Steven A. Wrighton, Peter J. A. Davies, David S. Loose, Gregory L. Shipley and Henry W. Strobel
Journal of Pharmacology and Experimental Therapeutics November 1, 2004, 311 (2) 728-735; DOI: https://doi.org/10.1124/jpet.104.068908

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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Estrogen Regulation of the Cytochrome P450 3A Subfamily in Humans

Eric T. Williams, Malgorzata Leyk, Steven A. Wrighton, Peter J. A. Davies, David S. Loose, Gregory L. Shipley and Henry W. Strobel
Journal of Pharmacology and Experimental Therapeutics November 1, 2004, 311 (2) 728-735; DOI: https://doi.org/10.1124/jpet.104.068908
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