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Journal of Pharmacology and Experimental Therapeutics

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Research ArticleNEUROPHARMACOLOGY

Ro 64-6198 [(1S,3aS)-8-(2,3,3a,4,5,6-Hexahydro-1H-phenalen-1-yl)-1-phenyl-1,3,8-triaza-spiro[4.5]decan-4-one] Acts Differently from Nociceptin/Orphanin FQ in Rat Periaqueductal Gray Slices

Lih-Chu Chiou, Kuang-Chieh Chuang, Juergen Wichmann and Geo Adam
Journal of Pharmacology and Experimental Therapeutics November 2004, 311 (2) 645-651; DOI: https://doi.org/10.1124/jpet.104.070219
Lih-Chu Chiou
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Kuang-Chieh Chuang
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Juergen Wichmann
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Geo Adam
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Abstract

Ro 64-6198 [(1S,3aS)-8-(2,3,3a,4,5,6-hexahydro-1H-phenalen-1-yl)-1-phenyl-1,3,8-triaza-spiro[4.5]decan-4-one] was developed as a nonpeptide agonist of nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptors, using bioassays at cloned receptors expressed in cell cultures. We have investigated the actions of Ro 64-6198 at native NOP receptors of the ventrolateral periaqueductal gray (PAG), a crucial site for N/OFQ-induced reversal of opioid analgesia, using the patch-clamp recording technique in rat brain slices. Ro 64-6198, like N/OFQ, activated G protein-coupled inwardly rectifying K+ channels (GIRK) in ventrolateral PAG neurons but displayed only 60% efficacy and 22% potency of N/OFQ. Unlike N/OFQ that activated GIRK through NOP receptors in almost all tested neurons, Ro 64-6198 affected only 62% (114/185) of the neurons recorded, among which 57% were sensitive to CompB (J-113397), a selective NOP receptor antagonist. The effect of Ro 64-6198 was not affected by naloxone (1 μM), sulpiride (10 μM), and [1-(2-methoxyphenyl)-4-[4-2-phthalimido)butyl]piperazine (NAN-190) (1 μM), respectively, the antagonist of opioid, dopamine D2, and 5-HT1A receptors. In Ro 64-6198-unresponsive neurons, N/OFQ activated GIRK through NOP receptors. It is concluded that Ro 64-6198 is a weak agonist of NOP receptors both in terms of potency and efficacy in ventrolateral PAG neurons. Heterogeneity of NOP receptors has been proposed from binding studies and in vivo functional studies. The possibility was discussed that two subsets of NOP receptors exist in ventrolateral PAG neurons, and Ro 64-6198 activates only one subset but N/OFQ activates both of them.

Footnotes

  • This study was supported by Grants NHRI-EX92-9005NC and NHRI-EX93-9005NC from the National Health Research Institutes and Grant NSC 92-2320-B002-088 from the National Science Council.

  • doi:10.1124/jpet.104.070219.

  • ABBREVIATIONS: N/OFQ, nociceptin/orphanin FQ; NOP, nociceptin/orphanin FQ peptide; Ro 64-6198, (1S,3aS)-8-(2,3,3a,4,5,6-hexahydro-1H-phenalen-1-yl)-1-phenyl-1,3,8-triaza-spiro[4.5]decan-4-one; PAG, periaqueductal gray; GIRK, G protein-coupled inwardly rectifying potassium channels; BAPTA, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid; ACSF, artificial cerebral spinal fluid; I-140, membrane current at -140 mV; I-V, current-voltage; NNC 63-0532, (8-naphthalen-1-ylmethyl-4-oxo-1-phenyl-1,3,8-triaza-spiro[4.5]dec-3-yl)-acetic acid methyl ester; NAN-190, 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine.

    • Received April 19, 2004.
    • Accepted July 8, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 311 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 311, Issue 2
1 Nov 2004
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Research ArticleNEUROPHARMACOLOGY

Ro 64-6198 [(1S,3aS)-8-(2,3,3a,4,5,6-Hexahydro-1H-phenalen-1-yl)-1-phenyl-1,3,8-triaza-spiro[4.5]decan-4-one] Acts Differently from Nociceptin/Orphanin FQ in Rat Periaqueductal Gray Slices

Lih-Chu Chiou, Kuang-Chieh Chuang, Juergen Wichmann and Geo Adam
Journal of Pharmacology and Experimental Therapeutics November 1, 2004, 311 (2) 645-651; DOI: https://doi.org/10.1124/jpet.104.070219

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Research ArticleNEUROPHARMACOLOGY

Ro 64-6198 [(1S,3aS)-8-(2,3,3a,4,5,6-Hexahydro-1H-phenalen-1-yl)-1-phenyl-1,3,8-triaza-spiro[4.5]decan-4-one] Acts Differently from Nociceptin/Orphanin FQ in Rat Periaqueductal Gray Slices

Lih-Chu Chiou, Kuang-Chieh Chuang, Juergen Wichmann and Geo Adam
Journal of Pharmacology and Experimental Therapeutics November 1, 2004, 311 (2) 645-651; DOI: https://doi.org/10.1124/jpet.104.070219
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