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Research ArticleTOXICOLOGY

Interaction between Ascorbate and Light-Exposed Riboflavin Induces Lung Remodeling

Jean-Claude Lavoie, Thérèse Rouleau and Philippe Chessex
Journal of Pharmacology and Experimental Therapeutics November 2004, 311 (2) 634-639; DOI: https://doi.org/10.1124/jpet.104.070755
Jean-Claude Lavoie
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Thérèse Rouleau
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Philippe Chessex
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Abstract

Light-exposed parenteral multivitamins induce in lungs peroxide-like oxidant responses as well as the initiation of fibrosis. We hypothesized that peroxides generated in light-exposed total parenteral nutrition (TPN) affect lung remodeling. The objective was to assess the specific roles of peroxides, multivitamin preparation (MVP), and light exposure on lung remodeling during TPN. Three-day-old guinea pigs fitted with an indwelling catheter were assigned to the following intravenous regimens: TPN or MVP ± photoprotection, H2O2± glutathione, MVP ± metabisulfite, or ascorbic acid ± riboflavin. Fed animals served as controls. After 4 days, lungs were sampled to determine alveolarization (intercepts), β-actin mRNA (protection assay), and apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labeling). Data were analyzed by analysis of variance. The infusion of light-exposed multivitamins induced a 20% lower (p < 0.01) alveolarization index than fed controls, and 3-fold higher (p < 0.01) apoptotic events. This was prevented by photoprotecting TPN. The effect of multivitamins on the alveolarization index was reproduced (p < 0.05) by infusion of light-exposed riboflavin in the presence of vitamin C. The alveolarization index correlated (r2 = 0.35; p < 0.05) with β-actin mRNA, suggesting alveolar disruption. Antiperoxides conferred no protection against decreased alveolarization. Lung remodeling induced by exposure of TPN to ambient light is not due to a direct effect of infused peroxides but rather to an interaction between vitamin C and peroxides generated by the exposure of riboflavin to light. It is speculated that this interaction may play a role in the development of chronic lung disease of premature infants who receive TPN and have immature antioxidant defenses.

Footnotes

  • This study was supported in part by institutional funding from the Research Centre of Hôpital Sainte-Justine and by Grant MOP 53270 from the Canadian Institutes of Health Research.

  • doi:10.1124/jpet.104.070755.

  • ABBREVIATIONS: TPN, total parenteral nutrition; MVP, multivitamin preparation; GSSG, glutathione disulfide; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; (-)light, photoprotected; (+)light, exposed to ambient light; BPD, broncho-pulmonary dysplasia.

    • Received April 29, 2004.
    • Accepted July 13, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 311 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 311, Issue 2
1 Nov 2004
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Research ArticleTOXICOLOGY

Interaction between Ascorbate and Light-Exposed Riboflavin Induces Lung Remodeling

Jean-Claude Lavoie, Thérèse Rouleau and Philippe Chessex
Journal of Pharmacology and Experimental Therapeutics November 1, 2004, 311 (2) 634-639; DOI: https://doi.org/10.1124/jpet.104.070755

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Research ArticleTOXICOLOGY

Interaction between Ascorbate and Light-Exposed Riboflavin Induces Lung Remodeling

Jean-Claude Lavoie, Thérèse Rouleau and Philippe Chessex
Journal of Pharmacology and Experimental Therapeutics November 1, 2004, 311 (2) 634-639; DOI: https://doi.org/10.1124/jpet.104.070755
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