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Research ArticleCELLULAR AND MOLECULAR

Massive Cell Vacuolization Induced by Organic Amines Such as Procainamide

Guillaume Morissette, Emmanuel Moreau, René C.-Gaudreault and François Marceau
Journal of Pharmacology and Experimental Therapeutics July 2004, 310 (1) 395-406; DOI: https://doi.org/10.1124/jpet.104.066084
Guillaume Morissette
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Emmanuel Moreau
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René C.-Gaudreault
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François Marceau
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Abstract

Procaine and some other basic drugs reportedly induce vacuolization of various cell types. We addressed the concentration-effect and structure-activity relationships as well as the mechanism of this effect using three cell lines. Massive vacuolization occurs over several hours in primary cultures of rabbit pulmonary artery smooth muscle cells (SMCs) and COS-1 cells in response to procaine and loosely related amine compounds (procainamide, N-acetyl-procainamide, metoclopramide, lidocaine, triethylamine, nicotine) used at 2.5 mM. Furthermore, chloroquine, propranolol, diphenhydramine, and neutral red are active in this respect at 100 to 250 μM in SMCs and COS-1 cells. Human embryonic kidney 293 cells mildly responded to triethylamine, nicotine, and propranolol only. Tetraethylammonium was uniformly inactive, as well as many other drugs in all three cell types (concentrations up to 2.5 mM). Procainamide does not induce apoptosis in SMCs treated for up to 48 h, although the vacuolization is sustained and proliferation and migration are reduced during this period. Procainamide-induced vacuolization is reversible on drug washing, largely prevented by bafilomycin A1 cotreatment, and has a tentatively identified Golgi origin (uptake of ceramide-C5). Procainamide and neutral red are concentrated in SMCs in a bafilomycin A1-sensitive manner. The preventive effect of bafilomycin A1 suggests that the vacuoles originate from the osmotic swelling of acidic organelles in which the charged basic drugs are trapped at low pH. Drug transport at the plasma membrane may be limiting for this type of response, as suggested by the cell type selectivity of agents and the inhibitory effect of some drugs such as quinidine.

Footnotes

  • This study was supported by the Canadian Institutes of Health Research Grant MOP-14077.

  • DOI: 10.1124/jpet.104.066084.

  • ABBREVIATIONS: OCT, organic cation transporter; LY294002, 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride; HEK, human embryonic kidney; HERG, human ether-a-go-go-related gene; SMC, smooth muscle cell; FBS, fetal bovine serum; EBSS, Earle's balanced salt solution; E-4031, N-[4-[[1-[2-(6-methyl-2-pyridinyl)ethyl]-4-piperidinyl]carbonyl]phenyl]-methanesulfonamide dihydrochloride.

    • Received January 26, 2004.
    • Accepted March 8, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 310 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 310, Issue 1
1 Jul 2004
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Research ArticleCELLULAR AND MOLECULAR

Massive Cell Vacuolization Induced by Organic Amines Such as Procainamide

Guillaume Morissette, Emmanuel Moreau, René C.-Gaudreault and François Marceau
Journal of Pharmacology and Experimental Therapeutics July 1, 2004, 310 (1) 395-406; DOI: https://doi.org/10.1124/jpet.104.066084

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Research ArticleCELLULAR AND MOLECULAR

Massive Cell Vacuolization Induced by Organic Amines Such as Procainamide

Guillaume Morissette, Emmanuel Moreau, René C.-Gaudreault and François Marceau
Journal of Pharmacology and Experimental Therapeutics July 1, 2004, 310 (1) 395-406; DOI: https://doi.org/10.1124/jpet.104.066084
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