Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleINFLAMMATION AND IMMUNOPHARMACOLOGY

Cloning and Pharmacological Characterization of CXCR1 and CXCR2 from Macaca fascicularis

R. William Hipkin, Gregory Deno, Jay Fine, Yongliang Sun, Brian Wilburn, Xuedong Fan, Waldemar Gonsiorek and Maria T. Wiekowski
Journal of Pharmacology and Experimental Therapeutics July 2004, 310 (1) 291-300; DOI: https://doi.org/10.1124/jpet.103.063131
R. William Hipkin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gregory Deno
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jay Fine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yongliang Sun
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brian Wilburn
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xuedong Fan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Waldemar Gonsiorek
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria T. Wiekowski
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Two genes with high sequence homology to human CXCR1 (hCXCR1) and CXCR2 (hCXCR2) were cloned from blood of cynomolgus monkey (Macaca fascicularis). Comparison of the expression pattern of these receptors in different species demonstrated that, like in humans, cynomolgus CXCR1 (cCXCR1) and CXCR2 (cCXCR2) are highly expressed in blood. Membranes from transfected BaF3 cells expressing cCXCR1 bind interleukin (IL)-8 with an affinity similar to hCXCR1 (Kd values, 170 ± 87 and 103 ± 37 pM, respectively) and show low binding affinity to Gro-α. Cynomolgus CXCR2 also binds hIL-8 but with somewhat higher affinity than the hCXCR2 (46 ± 28 and 220 ± 14 pM, respectively). Surprisingly, cCXCR2 has a reduced binding affinity to hGro-α (3.7 ± 2.2 nM), a specific ligand of hCXCR2 (540 ± 140 pM). Furthermore, the CXCR2-specific antagonist SB225002 [N-(2-hydroxy-4-nitrophenyl)-N′-(2-bromophenyl)urea] is 10-fold more potent in inhibiting IL-8 binding to hCXCR2 than to cCXCR2, suggesting that some of the observed differences in the amino acid sequences of the human and monkey receptor affect ligand binding sites or the conformation of the receptor. Both cynomolgus receptors were functionally active in inducing guanosine 5′-O-(3-thio)triphosphate exchange on membranes in response to IL-8 and Gro-α and in mediating chemotactic activity of recombinant BA/F3 cells in response to IL-8 and Gro-α. These results identify the products of the novel cynomolgus genes as functional homologs of hCXCR1 and hCXCR2.

Footnotes

  • DOI: 10.1124/jpet.103.063131.

  • ABBREVIATIONS: IL, interleukin; PCR, polymerase chain reaction; RACE, rapid amplification of cDNA ends; PBS, phosphate-buffered saline; BSA, bovine serum albumin; [35S]GTPγS, guanosine 5[prime]-O-(3-[35S]thio)triphosphate; SPA, scintillation proximity assay; WGA, wheat germ agglutinin; GTPγS, guanosine 5′-3-O-(thio)triphosphate; EL3, third extracellular loop; SB225002, N-(2-hydroxy-4-nitrophenyl)-N′-(2-bromophenyl)urea.

    • Received November 21, 2003.
    • Accepted March 16, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 310 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 310, Issue 1
1 Jul 2004
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Cloning and Pharmacological Characterization of CXCR1 and CXCR2 from Macaca fascicularis
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleINFLAMMATION AND IMMUNOPHARMACOLOGY

Cloning and Pharmacological Characterization of CXCR1 and CXCR2 from Macaca fascicularis

R. William Hipkin, Gregory Deno, Jay Fine, Yongliang Sun, Brian Wilburn, Xuedong Fan, Waldemar Gonsiorek and Maria T. Wiekowski
Journal of Pharmacology and Experimental Therapeutics July 1, 2004, 310 (1) 291-300; DOI: https://doi.org/10.1124/jpet.103.063131

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleINFLAMMATION AND IMMUNOPHARMACOLOGY

Cloning and Pharmacological Characterization of CXCR1 and CXCR2 from Macaca fascicularis

R. William Hipkin, Gregory Deno, Jay Fine, Yongliang Sun, Brian Wilburn, Xuedong Fan, Waldemar Gonsiorek and Maria T. Wiekowski
Journal of Pharmacology and Experimental Therapeutics July 1, 2004, 310 (1) 291-300; DOI: https://doi.org/10.1124/jpet.103.063131
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Lipopolysaccharide Induces Epithelium- and Prostaglandin E2-Dependent Relaxation of Mouse Isolated Trachea through Activation of Cyclooxygenase (COX)-1 and COX-2
  • Cannabinoid-Mediated Elevation of Intracellular Calcium: A Structure-Activity Relationship
  • Protease-Activated Receptor-2 Peptides Activate Neurokinin-1 Receptors in the Mouse Isolated Trachea
Show more INFLAMMATION AND IMMUNOPHARMACOLOGY

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics