Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleCARDIOVASCULAR

Atypical β-Adrenoceptor Subtypes Mediate Relaxations of Rabbit Corpus Cavernosum

Cleber E. Teixeira, Juliana S. Baracat, Angelina Zanesco, Edson Antunes and Gilberto De Nucci
Journal of Pharmacology and Experimental Therapeutics May 2004, 309 (2) 587-593; DOI: https://doi.org/10.1124/jpet.103.062026
Cleber E. Teixeira
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juliana S. Baracat
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Angelina Zanesco
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edson Antunes
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gilberto De Nucci
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

This study was performed to characterize the β-adrenoceptor population in rabbit isolated corpus cavernosum (RbCC) by using nonselective and selective β-adrenoceptor agonists and antagonists in functional assays. Metaproterenol, ritodrine, fenoterol, and 8-hydroxy-5-[(1R)-1-hydroxy-2-[N-[(1R)-2-(ρ-methoxyphenyl)-1-methylethyl]amino]ethyl]carbostyril (TA 2005) (3–100 nmol each) dose dependently relaxed the RbCC preparations. These relaxations were markedly reduced by Nω-nitro-l-arginine methyl ester (l-NAME; 10 μM) and 1H-[1,2,4]-oxadiazolo-[4,3,-a]quinoxalin-1-one (ODQ) (10 μM), whereas the adenylyl cyclase inhibitor SQ 22,536 [9-(2-tetrahydrofuryl) adenine] (10 μM) had no effect. In contrast, neither l-NAME nor ODQ affected the isoproterenol-induced RbCC relaxations, but SQ 22,536 abolished this response. Sildenafil (1 μM) significantly potentiated the relaxations induced by β2-agonists without affecting the isoproterenol-evoked relaxations. Rolipram (10 μM) enhanced the relaxations elicited by isoproterenol but had no effect on those induced by the selective β2 agonists. Propranolol and (±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]-2-butanol hydrochloride (ICI 118,551) determined a rightward shift in the concentration-response curves to isoproterenol in a noncompetitive manner with a reduction of maximum response at the highest antagonist concentration, with the slope values significantly different from unity. Propranolol and ICI 118,551 had no effect on the relaxations elicited by fenoterol, TA 2005, metaproterenol, and ritodrine. Atenolol and 1-[2-((3-carbamoyl-4-hydroxy)phenoxy) ethylamino]-3-[4-(1-methyl-4-trifluoromethyl-2-imidazolyl)-phenoxy]-2-propanol methanesulfonate (CGP 20712A) (0.1–10 μM) failed to affect the relaxations induced by all tested β-adrenoceptor agonists. Our study revealed the existence of two atypical β-adrenoceptors in the rabbit erectile tissue. Isoproterenol relaxes the rabbit cavernosal tissue by activating atypical β-adrenoceptors coupled to adenylyl cyclase pathway, whereas the selective β2-adrenoceptor agonists relax the RbCC tissue through another atypical β-adrenoceptor subtype coupled to nitric oxide release from the sinusoidal endothelium.

Footnotes

  • ABBREVIATIONS: RbCC, rabbit corpus cavernosum; TA 2005, 8-hydroxy-5-[(1R)-1-hydroxy-2-[N-[(1R)-2-(ρ-methoxy-phenyl)-1-methylethyl]amino]ethyl]carbostyril; BRL 37344, (±)-4-[2-[(2-(3-chlorophenyl)-2-hydroxyethyl)amino]propyl]phenoxyacetic acid; l-NAME, Nω-nitro-l-arginine methyl ester; ODQ, 1H-[1,2,4]-oxadiazolo-[4,3,-a]quinoxalin-1-one; SQ 22,536, 9-(2-tetrahydrofuryl) adenine; CGP 20712A (1-[2-((3-carbamoyl-4-hydroxy)phenoxy)ethylamino]-3-[4-(1-methyl-4-trifluoromethyl-2-imidazolyl)-phenoxy]-2-propanol methanesulfonate); ICI 118,551, (±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]-2-butanol hydrochloride; GTN, glyceryl trinitrate; NO, nitric oxide; ACh, acetylcholine; TTX, tetrodotoxin; PDE, phosphodiesterase.

  • C.E.T. is supported by Fundação de Amparo à Pesquisa do Estado de São Paulo.

  • Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.

  • DOI: 10.1124/jpet.103.062026.

    • Received October 23, 2003.
    • Accepted January 23, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 309 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 309, Issue 2
1 May 2004
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Atypical β-Adrenoceptor Subtypes Mediate Relaxations of Rabbit Corpus Cavernosum
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleCARDIOVASCULAR

Atypical β-Adrenoceptor Subtypes Mediate Relaxations of Rabbit Corpus Cavernosum

Cleber E. Teixeira, Juliana S. Baracat, Angelina Zanesco, Edson Antunes and Gilberto De Nucci
Journal of Pharmacology and Experimental Therapeutics May 1, 2004, 309 (2) 587-593; DOI: https://doi.org/10.1124/jpet.103.062026

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Research ArticleCARDIOVASCULAR

Atypical β-Adrenoceptor Subtypes Mediate Relaxations of Rabbit Corpus Cavernosum

Cleber E. Teixeira, Juliana S. Baracat, Angelina Zanesco, Edson Antunes and Gilberto De Nucci
Journal of Pharmacology and Experimental Therapeutics May 1, 2004, 309 (2) 587-593; DOI: https://doi.org/10.1124/jpet.103.062026
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Effect of Acetate and on HR, BP, and Cardiac Contractility
  • Amphetaminelike Effects of α-Ethylphenethylamine Analogs
  • Metoprolol Impairs Cerebral Vasodilator Responses
Show more Cardiovascular

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2021 by the American Society for Pharmacology and Experimental Therapeutics