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Research ArticleCELLULAR AND MOLECULAR

Identification of Novel Isoform-Selective Inhibitors within Class I Histone Deacetylases

Erding Hu, Edward Dul, Chiu-Mei Sung, Zunxuan Chen, Robert Kirkpatrick, Gui-Feng Zhang, Kyung Johanson, Ronggang Liu, Amparo Lago, Glenn Hofmann, Ricardo Macarron, Maite De Los Frailes, Paloma Perez, John Krawiec, James Winkler and Michael Jaye
Journal of Pharmacology and Experimental Therapeutics November 2003, 307 (2) 720-728; DOI: https://doi.org/10.1124/jpet.103.055541
Erding Hu
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Edward Dul
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Chiu-Mei Sung
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Zunxuan Chen
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Robert Kirkpatrick
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Gui-Feng Zhang
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Kyung Johanson
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Ronggang Liu
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Amparo Lago
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Glenn Hofmann
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Ricardo Macarron
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Maite De Los Frailes
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Paloma Perez
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John Krawiec
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James Winkler
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Michael Jaye
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Abstract

Histone deacetylases (HDACs) represent an expanding family of protein modifying-enzymes that play important roles in cell proliferation, chromosome remodeling, and gene transcription. We have previously shown that recombinant human HDAC8 can be expressed in bacteria and retain its catalytic activity. To further explore the catalytic activity of HDACs, we expressed two additional human class I HDACs, HDAC1 and HDAC3, in baculovirus. Recombinant HDAC1 and HDAC3 fusion proteins remained soluble and catalytically active and were purified to near homogeneity. Interestingly, trichostatin (TSA) was found to be a potent inhibitor for all three HDACs (IC50 value of ∼0.1-0.3 μM), whereas another HDAC inhibitor MS-27-275 (N-(2-aminophenyl)-4-[N-(pyridin-3-methyloxycarbonyl)-aminomethyl]benzamide) preferentially inhibited HDAC1 (IC50 value of ∼0.3 μM) versus HDAC3 (IC50 value of ∼8 μM) and had no inhibitory activity toward HDAC8 (IC50 value >100 μM). MS-27-275 as well as TSA increased histone H4 acetylation, induced apoptosis in the human colon cancer cell line SW620, and activated the simian virus 40 early promoter. HDAC1 protein was more abundantly expressed in SW620 cells compared with that of HDAC3 and HDAC8. Using purified recombinant HDAC proteins, we identified several novel HDAC inhibitors that preferentially inhibit HDAC1 or HDAC8. These inhibitors displayed distinct properties in inducing histone acetylation and reporter gene expression. These results suggest selective HDAC inhibitors could be identified using recombinantly expressed HDACs and that HDAC1 may be a promising therapeutic target for designing HDAC inhibitors for proliferative diseases such as cancer.

Footnotes

  • ↵1 Current address: Array BioPharma, 3200 Walnut St., Boulder, CO 80301.

  • DOI: 10.1124/jpet.103.055541.

  • ABBREVIATIONS: HAT, histone acetyltransferase; HDAC, histone deacetylase; TSA, trichostatin; GST, glutathione S-transferase; PCR, polymerase chain reaction; MTP, multiple titer plate; SV40, simian virus 40; PAGE, polyacrylamide gel electrophoresis; MS-27-275, N-(2-aminophenyl)-4-[N-(pyridin-3-methyloxycarbonyl)-aminomethyl]benzamide; FR901228, desipeptide; SB-499201, N-(4-phenoxyphenyl)-4-(3-phenylpropanoylamino)-benzamide; SB-379872-A, 4-pyridin-4-yl-1,3-imidazole-2-thione.

    • Received June 10, 2003.
    • Accepted August 7, 2003.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 307 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 307, Issue 2
1 Nov 2003
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Research ArticleCELLULAR AND MOLECULAR

Identification of Novel Isoform-Selective Inhibitors within Class I Histone Deacetylases

Erding Hu, Edward Dul, Chiu-Mei Sung, Zunxuan Chen, Robert Kirkpatrick, Gui-Feng Zhang, Kyung Johanson, Ronggang Liu, Amparo Lago, Glenn Hofmann, Ricardo Macarron, Maite De Los Frailes, Paloma Perez, John Krawiec, James Winkler and Michael Jaye
Journal of Pharmacology and Experimental Therapeutics November 1, 2003, 307 (2) 720-728; DOI: https://doi.org/10.1124/jpet.103.055541

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Research ArticleCELLULAR AND MOLECULAR

Identification of Novel Isoform-Selective Inhibitors within Class I Histone Deacetylases

Erding Hu, Edward Dul, Chiu-Mei Sung, Zunxuan Chen, Robert Kirkpatrick, Gui-Feng Zhang, Kyung Johanson, Ronggang Liu, Amparo Lago, Glenn Hofmann, Ricardo Macarron, Maite De Los Frailes, Paloma Perez, John Krawiec, James Winkler and Michael Jaye
Journal of Pharmacology and Experimental Therapeutics November 1, 2003, 307 (2) 720-728; DOI: https://doi.org/10.1124/jpet.103.055541
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